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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
Overview
Product name
Anti-Adenosine Receptor A2a antibody
See all Adenosine Receptor A2a products (7) ...
Description
Rabbit polyclonal to Adenosine Receptor A2a
Specificity
Detects adenosine receptor A2a. This antibody does not detect other AR subtypes.
Tested applications
WB, IHC-P, IP, ICC/IFmore details
Cross reactivity
Reacts with
Dog, Human
Immunogen
Synthetic peptide: ESHGDMGLPDVELLSHELK, corresponding to amino acids 373-391 of Dog Adenosine Receptor A2a.
ESHGDMGLPD VELLSHELK
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Cell Biology >> Apoptosis >> Phagocytosis
Cardiovascular >> Blood >> Coagulation >> Regulatory
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
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Immunocytochemistry/ Immunofluorescence - Anti-Adenosine Receptor A2a antibody (ab3461)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab3461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
WB: 1/1000WB: 1/1000
IHC-P
IHC-P: 1/100IHC-P: 1/100
IP: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Domain
The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.
Post-translational
modifications
Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP29274
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A2AAR antibody
- A2aR antibody
- AA2AR_HUMAN antibody
see all
Database links
- Entrez Gene: 135 Human
- Omim: 102776 Human
- SwissProt: P11617 Dog
- SwissProt: P29274 Human
- Unigene: 197029 Human
Anti-Adenosine Receptor A2a antibody images:
Immunocytochemistry/ Immunofluorescence - Anti-Adenosine Receptor A2a antibody (ab3461)
ICC/IF image of ab3461 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3461, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Adenosine Receptor A2a antibody (ab3461)
This product has been referenced in:
- Buira SPet al. DNA methylation regulates adenosine A(2A) receptor cell surface expression levels. J Neurochem 112:1273-85 (2010). WB; Human.Read more (PubMed: 20002525) »
- Datino Tet al. Mechanisms by which adenosine restores conduction in dormant canine pulmonary veins. Circulation 121:963-72 (2010).Read more (PubMed: 20159830) »
See all 2 publications for this product
Publishing research using ab3461? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"