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Overview

  • Product nameAnti-Adenovirus E1A antibody [0.N.7]See all Adenovirus E1A primary antibodies ...
  • Description
    Mouse monoclonal [0.N.7] to Adenovirus E1A
  • SpecificityThis antibody is recommended for detection of E1A antigens of Adenovirus-2 and Adenovirus-5 origin.
  • Tested applicationsIHC-P, WB, IP, ICC/IF more details
  • Immunogen

    Adenovirus.

Properties

Applications

Our Abpromise guarantee covers the use of ab33183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use at an assay dependent dilution.
WB WB: Use a concentration of 1 µg/ml.
IP IP: Use a concentration of 2 µg/ml.
ICC/IF ICC/IF: Use at an assay dependent dilution.

Target

  • RelevanceThe early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.
  • Cellular localizationNuclear
  • Alternative names
      Control protein E1A antibodyE1A antibody

Anti-Adenovirus E1A antibody [0.N.7] images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Adenovirus E1A antibody [0.N.7] (ab33183)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Adenovirus E1A antibody [0.N.7] (ab33183)
    Adenovirus infected Human appendix stained with ab33183 and DAB Chromagen.
  • Western blot - Adenovirus E1A antibody [0.N.7] (ab33183)
    Western blot - Adenovirus E1A antibody [0.N.7] (ab33183)
    20ug cell lysate (cell line derived from Human Renal Cancer-2), primary antibody dilution is 1ug/ml, overnight incubation at 4°C.

References for Anti-Adenovirus E1A antibody [0.N.7] (ab33183)

This product has been referenced in:
  • Cawood R  et al. MicroRNA Controlled Adenovirus Mediates Anti-Cancer Efficacy without Affecting Endogenous MicroRNA Activity. PLoS One 6:e16152 (2011). WB . Read more (PubMed: 21264344) »
  • Günther PS  et al. CD209/DC-SIGN mediates efficient infection of monocyte-derived dendritic cells by clinical adenovirus 2C isolates in the presence of bovine lactoferrin. J Gen Virol 92:1754-9 (2011). ICC/IF . Read more (PubMed: 21562123) »

See all 3 Publications for this product

Publishing research using ab33183 ? Please let us know so that we can cite the reference in this datasheet

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Displaying 1 - 4 of 4 results for Abreviews and Q&A

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Votes: Highest First

Dear Sir or Madam,

would you please send me a Certificate of Analysis and Certificate of Origin for ab33183 Lot: GR65460 and ab58066 Lot: GR51251? Thank you!

Kind regards,

Thank you for your enquiry.

Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Ab...

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I need a certificate of analysis, Certificate of origin and safety data sheet for E1A antibody ab33183, Lot: XXXXX. Would you be so kind to send me these?
Thank you!

Vielen Dank für Ihre Anfrage.

Unsere MSDS finden Sie, sofern es sich um ein Produkt mit gesundheitsschädlichen Inhaltsstoffen handelt, immer als PDFDatei auf unserem Datenblatt in der rechten Ecke unter der PDF Datei des Datenblattes....

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Dear Sir or Madam,

please send me a Certificate of Analysis, Certificate of Origin and Safety Data Sheet for ab33183.

Thank you!
Kind regards,

Thank you for your request.

Please find the Certificate of Compliance (CoC) for the lot GR56563-1 of ab33183 in the attachment.

The Material Safety Datasheet (MSDS) can be downloaded directly from the online datasheet through the link ...

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Can you tell me more about the WB image?

Thank you for your enquiry. This antibody recognizes three adenovirus (Ad) 2 and 5 E1A gene products, with bands in the range 36-50kD. Different Ad2 infected cell lines can display one or more multiple bands. The Western Blot image provided used ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"