The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes IHC-P: Working dilution: Ready-to-use for 30 min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
Hadv 5 E1A antibody
HAdv5 E1A antibody
References for Anti-Adenovirus Type 5 E1A antibody [SPM230], prediluted (ab17803)