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Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted.
Additional information regarding Adenovirus type 5 capsid proteins.
ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4ºC. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37ºC. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.
Image courtesy of an anonymous Abreview.
All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
Lane 1 : Whole cell lysate prepared from U2OS cells.
Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.
Lysates/proteins at 35 µg per lane.
Secondary
Goat anti-rabbit conjugated to IRDye 700DX
Blocking step was performed using 5% milk for 1 hour at 23°C.
Image courtesy of an anonymous Abreview.
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