Products:Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Adenovirus
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Our customer would like to purchase adenovirus antibody which reacts to serotype 5. |
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ANSWER: |
Thank you for contacting us. |
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do you have anti-adenovirus antibody which have the same specificity as ab6982 but biotin-labelled? |
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ANSWER: |
Thank you for contacting us. |
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Phone call: Customer kindly asked for the actual concentration of anti-Adenovirus Type 5 antibody. |
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ANSWER: |
Thank very much for your patience. This antibody has been developed as a serum; it is not purified further. We are unable to provide the purified version of this product. We also can’t purify it as a custom request. I am sorry we are unable to fulfil your request this time. We are however ready to provide any additional information you may need which can help you to develop your experiment. I hope this information will be helpful. Should you any further questions please do not hesitate to ask? |
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Phone call: Customer kindly asked for the actual concentration of anti-Adenovirus Type 5 antibody. |
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ANSWER: |
Thank you for contacting us. I can confirm that I have forwarded your request to my colleague in lab and now I am waiting their reply. We currently aren’t shipping due to Christmas holidays. The next shipping day will be 3rd January 2012. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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I am requesting documentation stating that the rabbit used in cat# ab6982 is or is not SPF (Specific Pathogen Free). |
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ANSWER: |
The rabbits used for production of ab6982 have not been certified as SPF (specific pathogen-free).
Please let me know if you have any other questions. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted.
Additional information regarding Adenovirus type 5 capsid proteins.
ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4ºC. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37ºC. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.
Image courtesy of an anonymous Abreview.
All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
Lane 1 : Whole cell lysate prepared from U2OS cells.
Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.
Lysates/proteins at 35 µg per lane.
Secondary
Goat anti-rabbit conjugated to IRDye 700DX
Blocking step was performed using 5% milk for 1 hour at 23°C.
Image courtesy of an anonymous Abreview.
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