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Products:Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Adenovirus
Overview
Product name
Anti-Adenovirus Type 5 antibody
See all Adenovirus Type 5 products (2) ...
Description
Rabbit polyclonal to Adenovirus Type 5
Specificity
Targets all capsid proteins of Adenovirus type 5, including hexon, fiber and penton. Cross reacts with other serotypes of Adenovirus.
Tested applications
Neutralising, IHC-P, IHC-Fr, ELISA, WB, ICC/IFmore details
Immunogen
Purified Adenovirus type 5
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Adenovirus
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Neut: Use at an assay dependent dilution. (This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml).)
IHC-P: Use at an assay dependent dilution. (PubMed: 20442708)
IHC-Fr: Use at an assay dependent dilution. (PubMed: 20442708)
ELISA: Use at an assay dependent dilution. (Recognizes Ad5 immobilized on microtiter plates so it should work in a capture or detection mode.)
WB: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
Target
Relevance
Adenoviruses are DNA viruses that cause upper respiratory tract infections, conjunctivitis, and other infections in humans. Adenoviruses were discovered in 1953. About 47 different types have been identified since then, and about half of them are believed to cause human diseases. Infants and children are most commonly affected by adenoviruses. Adenovirus infections can occur throughout the year, but seem to be most common from fall to spring. Adenoviruses are responsible for 3-5% of acute respiratory infections in children and 2% of respiratory illnesses in adults.
Alternative names
- Adenovirus 5 antibody
- AdV 5 antibody
Anti-Adenovirus Type 5 antibody images:
Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted.
- Adenovirus Type 5 antibody (ab6982)
Additional information regarding Adenovirus type 5 capsid proteins.
Immunocytochemistry/ Immunofluorescence - Adenovirus Type 5 antibody (ab6982)
ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.
Image courtesy of an anonymous Abreview.
Western blot - Adenovirus Type 5 antibody (ab6982)
All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
Lane 1 : Whole cell lysate prepared from U2OS cells.
Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.
Lysates/proteins at 35 µg per lane.
Secondary
Goat anti-rabbit conjugated to IRDye 700DX
Blocking step was performed using 5% milk for 1 hour at 23°C.
Image courtesy of an anonymous Abreview.
References for Anti-Adenovirus Type 5 antibody (ab6982)
This product has been referenced in:
- Ross PJet al. Assembly of helper-dependent adenovirus DNA into chromatin promotes efficient gene expression. J Virol 85:3950-8 (2011).Read more (PubMed: 21307207) »
- Guedan Set al. Hyaluronidase expression by an oncolytic adenovirus enhances its intratumoral spread and suppresses tumor growth. Mol Ther 18:1275-83 (2010). IHC-P, IHC-Fr.Read more (PubMed: 20442708) »
See all 19 publications for this product
Publishing research using ab6982? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"