Anti-Adenovirus antibody [B025 / AD51] (ab7428)
Key features and details
- Mouse monoclonal [B025 / AD51] to Adenovirus
- Suitable for: IHC-P, ELISA, IHC-Fr
- Reacts with: Adenovirus
- Isotype: IgG2a
Overview
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Product name
Anti-Adenovirus antibody [B025 / AD51]
See all Adenovirus primary antibodies -
Description
Mouse monoclonal [B025 / AD51] to Adenovirus -
Host species
Mouse -
Specificity
This antibody recognises all Adenovirus serotypes; binding to the hexon polypeptide. No cross-reactions are known. -
Tested applications
Suitable for: IHC-P, ELISA, IHC-Frmore details -
Species reactivity
Reacts with: Adenovirus -
Immunogen
Tissue, cells or virus corresponding to Adenovirus Adenovirus. Adenovirus type 3 (ATCC strain VR847)
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General notes
With recent studies indicating that viral agents may be implicated in new emerging diseases reported around the world, interest in viruses has increased and encompassed many different viral genera. The adenoviruses are represented by several different groups yet there are only few monoclonal antibodies that are commercially available that recognise all known serotypes.Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant -
Primary antibody notes
Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease. -
Clonality
Monoclonal -
Clone number
B025 / AD51 -
Myeloma
JK.Ag8.653 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000.
This product requires protein digestion pre-treatment of paraffin sections (e.g. trypsin or pronase) for 15 min prior to primary incubation |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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IHC-P
1/1000. This product requires protein digestion pre-treatment of paraffin sections (e.g. trypsin or pronase) for 15 min prior to primary incubation |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Relevance
Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease. -
Alternative names
- Adenovirus antibody
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab7428 has been referenced in 8 publications.
- Michalik S et al. Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines. Haematologica 107:947-957 (2022). PubMed: 35045692
- Chen Q et al. Clinical Features, Replication Competence, and Innate Immune Responses of Human Adenovirus Type 7 Infection. J Infect Dis 223:1390-1399 (2021). PubMed: 32840612
- Greinacher A et al. Insights in ChAdOx1 nCoV-19 vaccine-induced immune thrombotic thrombocytopenia. Blood 138:2256-2268 (2021). PubMed: 34587242
- Vierboom MPM et al. Evaluation of heterologous prime-boost vaccination strategies using chimpanzee adenovirus and modified vaccinia virus for TB subunit vaccination in rhesus macaques. NPJ Vaccines 5:39 (2020). PubMed: 32435513
- Storm RJ et al. Human Adenovirus Type 37 Uses aVß1 and a3ß1 Integrins for Infection of Human Corneal Cells. J Virol 91:N/A (2017). PubMed: 27974569
- Marschall M et al. Assessment of drug candidates for broad-spectrum antiviral therapy targeting cellular pyrimidine biosynthesis. Antiviral Res 100:640-8 (2013). PubMed: 24149002
- Iacovides DC et al. Critical role for arginine methylation in adenovirus-infected cells. J Virol 81:13209-17 (2007). PubMed: 17686851
- De Jong JC et al. Adenoviruses from human immunodeficiency virus-infected individuals, including two strains that represent new candidate serotypes Ad50 and Ad51 of species B1 and D, respectively. J Clin Microbiol 37:3940-5 (1999). PubMed: 10565911