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Adenylate Kinase 1 protein (Tagged-His Tag) (ab91894)

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Overview

  • Product nameAdenylate Kinase 1 protein (Tagged-His Tag)See all Adenylate Kinase 1 proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 2-194 of Human Adenylate Kinase 1 with a N terminal His tag; predicted MWt 22kDa: Uniprot ID = P00568.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 151 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGD LLRSEVSSGSARGK KLSEIMEKGQLVPLETVLDMLRDA MVAKVNTSKGFLIDGYPREVQQGEEFERRI GQPTLLLY VDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKAT EPVIAF YEKRGIVRKVNAEGSVDSVFSQVCTHLDALK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91894 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Adenylate kinase 1Adenylate kinase isoenzyme 1Adenylate kinase soluble
        AK 1AK1ATP AMP transphosphorylaseATP-AMP transphosphorylase 1KAD1_HUMANMyokinase
      see all
  • FunctionCatalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
  • Involvement in diseaseDefects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency [MIM:612631].
  • Sequence similaritiesBelongs to the adenylate kinase family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P00568 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Adenylate Kinase 1 protein (Tagged-His Tag) images

    • SDS-PAGE - Adenylate Kinase 1 protein (Tagged-His Tag) (ab91894)
      SDS-PAGE - Adenylate Kinase 1 protein (Tagged-His Tag) (ab91894)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Adenylate Kinase 1 protein (Tagged-His Tag) (ab91894)

    ab91894 has not yet been referenced specifically in any publications.

    Publishing research using ab91894 ? Please let us know so that we can cite the reference in this datasheet

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