Overview

  • Product name
    Adipogenesis Assay Kit (Cell-Based)
    See all Adipogenesis kits
  • Sample type
    Adherent cells
  • Assay type
    Cell-based
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Adipogenesis Assay Kit (ab133102) provides the reagents required for studying the induction and inhibition of adipogenesis in the established 3T3-L1 model using the adipogenesis induction procedure. This kit can also be used to screen drug candidates involved in adipogenesis. The classic Oil Red O staining for lipid droplets is used in this kit as an indicator of the degree of adipogenesis and can be quantified with a plate reader after the dye is conveniently extracted from the lipid droplet.

  • Notes

    Obesity is a risk factor in many of the major chronic diseases such as diabetes mellitus, and cancer. The ability to regulate the number of fat cells and/or the size of fat cells is a key in the development and physiology of obesity and also in the origin of chronic disease. Mammals have both brown adipose tissue (BAT), which utilizes lipids to generate heat in a process known as thermogenesis, and white adipose tissue (WAT) which stores excess energy as triglycerides in lipid droplets. Adipocytes are derived from multipotent mesenchymal precursor cells commit to preadipocytes and then either remain dormant or proceed to become differentiated adipocytes.

    3T3-L1 cells are a well-characterized cell line to study the differentiation of adipocytes, and other mechanisms such as insulin-induced glucose uptake and mechanisms of obesity development. This model system has greatly advanced the understanding of the molecular basis and signaling pathways of adipogenesis. During terminal differentiation, the fibroblast-like preadipocytes undergo a series of morphological and biochemical changes to eventually accumulate lipid droplets. This in vitro differentitated adipocytes share similar morphology with adipocytes in vivo.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab133102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Lipid droplet accumulation in the differentiated 3T3-L1 cells visualized by Oil Red O Solution staining.

  • Upon induction, 3T3-L1 cells differentiate into adipocytes-like cells and they are ready to be used for adipogenesis experiments.
    Panel A: Non-differentiated 3T3-L1 cells were not stained by Oil Red O Solution.

    Panel B: more than 80% of preadipocytes were differentiated four days after weaning the cells from induction medium to insulin medium. Lipid droplet accumulation in the differentiated cells can be visualized by Oil Red O Solution staining.

Protocols

References

ab133102 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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