For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Adipogenesis Assay Kit (ab133102) provides the reagents required for studying the induction and inhibition of adipogenesis in the established 3T3-L1 model using the adipogenesis induction procedure. This kit can also be used to screen drug candidates involved in adipogenesis. The classic Oil Red O staining for lipid droplets is used in this kit as an indicator of the degree of adipogenesis and can be quantified with a plate reader after the dye is conveniently extracted from the lipid droplet.
Obesity is a risk factor in many of the major chronic diseases such as diabetes mellitus, and cancer. The ability to regulate the number of fat cells and/or the size of fat cells is a key in the development and physiology of obesity and also in the origin of chronic disease. Mammals have both brown adipose tissue (BAT), which utilizes lipids to generate heat in a process known as thermogenesis, and white adipose tissue (WAT) which stores excess energy as triglycerides in lipid droplets. Adipocytes are derived from multipotent mesenchymal precursor cells commit to preadipocytes and then either remain dormant or proceed to become differentiated adipocytes.
3T3-L1 cells are a well-characterized cell line to study the differentiation of adipocytes, and other mechanisms such as insulin-induced glucose uptake and mechanisms of obesity development. This model system has greatly advanced the understanding of the molecular basis and signaling pathways of adipogenesis. During terminal differentiation, the fibroblast-like preadipocytes undergo a series of morphological and biochemical changes to eventually accumulate lipid droplets. This in vitro differentitated adipocytes share similar morphology with adipocytes in vivo.
|Adipogenesis Assay Dexamethasone Solution||1 x 500µl|
|Adipogenesis Assay Insulin Solution (1,000X)||1 x 1.5ml|
|Cell Based Assay IBMX Solution (1,000X)||1 x 500µl|
|Fixative (10X)||1 x 10ml|
|Lipid Droplets Assay Dye Extraction Solution||1 x 30ml|
|Lipid Droplets Assay Oil Red O Solution||1 x 25ml|
|Lipid Droplets Assay Wash Solution||6 x 30ml|
Our Abpromise guarantee covers the use of ab133102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Lipid droplet accumulation in the differentiated 3T3-L1 cells visualized by Oil Red O Solution staining.
ab133102 has not yet been referenced specifically in any publications.