Overview

  • Product nameAdipogenesis Detection KitSee all Adipogenesis kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture extracts, Tissue, Adherent cells, Suspension cells, Tissue Extracts, Cell Lysate
  • Assay typeQuantitative
  • Sensitivity
    > 0.2 nM
  • Range
    0.2 nM - 10 nM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Adipogenesis Detection Kit quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect triglyceride in as few as a thousand or less differentiated 3T3-L1 cells with triglyceride detection linear range 0.2 to 10 nmol. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or inhibitors, or to screen drugs.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceAdipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.

Applications

Our Abpromise guarantee covers the use of ab102513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

Adipogenesis Detection Kit images

  • Example of a standard curve obtained with ab102513.

  • Example of a standard curve obtained with ab102513.

Protocols

References for Adipogenesis Detection Kit (ab102513)

ab102513 has not yet been referenced specifically in any publications.

Product Wall



In our hands, ˜1,000-10,000 differentiated 3T3 cells using 100ul Lipid Extraction Solution are sufficient for the colorimetric assay. Then we use 5-50ul of the extract for the assay. I would start with 5ul of the lipid extract for the assay. ...

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The lipid extraction buffer can be thawed at RT.

Ibelieve the standard can be made available separately fromthe kit. If it is these things usually take 1-2 weeks to become available for purchase. And since itis a new product it would take 1-2...

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ab102513
Sample types: Cell and Tissue culture supernatants
Lipid extraction performed: The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers ...

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Thank you for calling Abcam.

The sample volume number that you use is the volume that you originally added to the reaction at stage 1 (add 5-50ul of sample). To normalize the data, you can either divide the value you get by the number of cell...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry about the problems caused by this product. It is a good rule of thumb to always thoroughly rea...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"