Overview
- Product nameAdipogenesis Detection KitSee all Adipogenesis kits ...
- Tests100 x 1 assay
- Sample typeCell culture extracts, Tissue, Adherent cells, Suspension cells, Tissue Extracts, Cell Lysate
- Assay typeQuantitative
- Sensitivity> 0.2 nM
- Range0.2 nM - 10 nM
- Assay time0h 40m
- Product overview
Abcam's Adipogenesis Detection Kit quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect triglyceride in as few as a thousand or less differentiated 3T3-L1 cells with triglyceride detection linear range 0.2 to 10 nmol. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or inhibitors, or to screen drugs.
Visit our FAQs page for tips and troubleshooting. - Notes
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 100 tests Adipogenesis Assay Buffer WM 1 x 25ml Adipogenesis Enzyme Mix Green 1 vial Adipogenesis Probe (in DMSO solution) Red 1 x 200µl Lipase Blue 1 vial Lipid Extraction Solution NM 1 x 10ml Triglyceride Standard Yellow 1 x 0.3ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab102513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Adipogenesis Detection Kit images
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Functional Studies - Adipogenesis Detection Kit (ab102513)Example of a standard curve obtained with ab102513
Protocols
References for Adipogenesis Detection Kit (ab102513)
ab102513 has not yet been referenced specifically in any publications.
