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Overview

  • Product nameAdiponectin Human ELISA KitSee all Adiponectin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 0.7 ng/ml
  • Range
    0.781 ng/ml - 50 ng/ml
  • Recovery

    101 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Adiponectin, also known as Adipocyte Complement-Related Protein of 30kDa (ACRP30), is a secreted serum protein expressed exclusively in differentiated adipocytes. Studies indicated that decreased plasma adiponectin concentration is associated with obesity, insulin resistance, essential hypertension, inflammation and atherosclerosis, and acute myocardial infarction. On the other hand, increased adiponectin level leads to nephrotic syndrome.

    ab108786 Adiponectin Human ELISA kit is designed for detection of adiponectin in human urine, plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures adiponectin in less than 4 hours. A polyclonal antibody specific for adiponectin has been pre-coated onto a microplate. Adiponectin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for adiponectin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

     

    This assay recognizes both natural and recombinant human adiponectin. It can detect both globular domain and full-length adiponectin.

  • Tested applicationsELISA more details

Properties

  • FunctionReceptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin.
  • Tissue specificityWidely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine.
  • Sequence similaritiesBelongs to the ADIPOR family.
  • DomainThe N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.
  • Cellular localizationMembrane. Localized to the cell membrane and intracellular organelles.

    • Target information above from: UniProt accession Q96A54 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        30 kDa adipocyte complement related protein ACDCACRP 30
        ACRP30 Adipocyte C1q and collagen domain containing protein Adipocyte complement related 30 kDa protein Adipocyte complement related protein of 30 kDaAdiponectin receptor protein 1AdipoQAdipor1Adipose most abundant gene transcript 1ADR1_HUMANAPM 1ApM1 GBP 28 GBP28 Gelatin binding protein 28OTTHUMP00000210047Progestin and adipoQ receptor family member I
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108786 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Adiponectin Human ELISA Kit images

      • ELISA - Adiponectin Human ELISA Kit (ab108786)
        ELISA - Adiponectin Human ELISA Kit (ab108786)
        Human Adiponectin Standard Curve

      Protocols

      References for Adiponectin Human ELISA Kit (ab108786)

      ab108786 has not yet been referenced specifically in any publications.

      Publishing research using ab108786 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab108786 Submit a Question for ab108786

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      Votes: Highest First

      Je voulais avoir quelques informations. Nous avons reçu un kit ELISA pour l’adiponectine. Malheureusement la technicienne l’a mis à –20°C. Pensez vous que nous pouvons encore réaliser les mesures...

      Merci de nous avoir contactés.

      Je pense que le kit est toujours utilisable si il n'est pas resté trop longtemps à -20ºC. Comme indiqué dans le livret fourni avec le kit, certains produits peuvent être stock...

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      Dear Sir/Madam Please kindly let me know for coating antibody of ab99968 and ab108786 are monoclonal ab or polyclonal ab.

      Thank you for contacting us. The capture antibody for the Adiponectin Human ELISA Kits are a monoclonal mouse antibody for kit ab99968 and a polyclonal antibody for kit ab108786 I hope this information is helpful to you. Please do not hesi...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"