Adiponectin Mouse ELISA kit (ab108785)
- Product nameAdiponectin Mouse ELISA kitSee all Adiponectin kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 4.8%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Urine, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity= 0.7 ng/ml
- Range0.78 ng/ml - 50 ng/ml
- Assay time4h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Mouse
- Product overview
Abcam’s Adiponectin Mouse in vitro ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of Adiponectin levels in urine, plasma, serum and cell culture supernatants.
An Adiponectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Adiponectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Adiponectin captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Mouse Adiponectin Antibody 1 x 140µl Adiponectin Microplate (12 x 8 well strips) 1 unit Adiponectin Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
- FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
- Tissue specificitySynthesized exclusively by adipocytes and secreted into plasma.
- Involvement in diseaseAdiponectin deficiency (ADPND) [MIM:612556]: A condition that results in very low concentrations of plasma adiponectin. Note=The disease is caused by mutations affecting the gene represented in this entry.
Diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]: A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
- Sequence similaritiesContains 1 C1q domain.
Contains 1 collagen-like domain.
- DomainThe C1q domain is commonly called the globular domain.
modificationsHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
- Cellular localizationSecreted.
- 30 kDa adipocyte complement related protein
- 30 kDa adipocyte complement-related protein
- Adipocyte C1q and collagen domain containing protein
- Adipocyte complement related 30 kDa protein
- Adipocyte complement related protein of 30 kDa
- Adipocyte complement-related 30 kDa protein
- Adipose most abundant gene transcript 1
- Adipose most abundant gene transcript 1 protein
- Adipose specific collagen like factor
- C1q and collagen domain-containing protein
- Gelatin binding protein
- Gelatin binding protein 28
- Gelatin-binding protein
Our Abpromise guarantee covers the use of ab108785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Adiponectin Mouse ELISA kit (ab108785)
ab108785 has not yet been referenced specifically in any publications.