Adiponectin Receptor 1 antibody - Carboxyterminal end (ab53398)

Product Name 

Adiponectin Receptor 1 antibody - Carboxyterminal end

Product type 

Primary antibodies

Description 

Goat polyclonal to Adiponectin Receptor 1 - Carboxyterminal end

Immunogen 

25 amino acid synthetic peptide VAAAFVHFYGVSNLQEFRYGLEGGC corresponding to aa 345-369 of the C-terminal domain of Human Adiponectin Receptor 1. Peptide availabe as ab88858 - Adiponectin Receptor 1 peptide (345-369)

Note: Peptide sequence is < 50 % identical to other receptors in this region.
BLAST 'VAAAFVHFYGVSNLQEFRYGLEGGC' with or

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: 1/400.
IHC-P: 1/250.
WB: 1/1000. Predicted molecular weight: 42 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human liver; human muscle.

Cellular localization 

Multipass membrane protein. Localized to the cell membrane and intracellular organelles.

Research areas 

Signal Transduction >> Growth Factors/Hormones >> Hormones
Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Acrp

Relevance 

Acrp30 is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR- . Cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) have shown that AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. These two adiponectin receptors are predicted to contain seven transmembrane domains, but are structurally and functionally distinct from G-protein-coupled receptors. Expression of AdipoR1/R2 or suppression of AdipoR1/R2 expression by small-interfering RNA supports the conclusion that they serve as receptors for globular and full-length adiponectin, and that they mediate increased AMP kinase and PPAR- ligand activities, as well as fatty-acid oxidation and glucose uptake by adiponectin. Widely expressed. Highly expressed in skeletal muscle. ADR 1 is expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1mg/ml BSA, 140mM Sodium chloride, 10mM Potassium phosphate, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

AdipoR1 is highly conserved in species ranging from microorganisms to humans, and ab53398 recognizes AdipoR1 in multiple species.

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