Anti-Adiponectin antibody (ab59687)

Overview

• Product nameAnti-Adiponectin antibodySee all Adiponectin primary antibodies ...
• Description
  Chicken polyclonal to Adiponectin
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Rabbit, Cow, Cat, Dog, Rhesus monkey
• Immunogen

  Synthetic peptide: NDSTFTGFLLYHDTN, corresponding to amino acids 230 - 244 of Human Adiponectin

  Run BLAST with Run BLAST with
• Positive controlMouse plasma

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgY
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Types of disease
  • Diabetes

  • Metabolism
  • Types of disease
  • Metabolic disorders

  • Stem Cells
  • Mesenchymal Stem Cells
  • Adipogenesis

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Signal Transduction
  • Growth Factors/Hormones
  • Hormones

  • Cardiovascular
  • Lipids / Lipoproteins
  • Adipose Related
  • Acrp

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Neuroscience
  • Neurology process
  • Metabolism

Applications

Target

• FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
• Tissue specificitySynthesized exclusively by adipocytes and secreted into plasma.
• Involvement in diseaseDefects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
  Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
• Sequence similaritiesContains 1 C1q domain.
  Contains 1 collagen-like domain.
• DomainThe C1q domain is commonly called the globular domain.
• Post-translational
  modificationsHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
  HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
  O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
• Cellular localizationSecreted.

Target information above from: UniProt accession Q15848 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 282865 Cow
  • Entrez Gene: 403625 Dog
  • Entrez Gene: 9370 Human
  • Entrez Gene: 11450 Mouse
  • Entrez Gene: 246253 Rat
  • Omim: 605441 Human
  • SwissProt: Q15848 Human
  • SwissProt: Q60994 Mouse

  • Unigene: 80485 Human
  • Unigene: 3969 Mouse

  see all

• Alternative names
  30 kDa adipocyte complement related protein antibody30 kDa adipocyte complement-related protein antibodyACDC antibody

  ACRP 30 antibodyACRP30 antibodyADIPO_HUMAN antibodyAdipocyte antibodyAdipocyte C1q and collagen domain containing protein antibodyAdipocyte complement related 30 kDa protein antibodyAdipocyte complement related protein of 30 kDa antibodyAdipocyte complement-related 30 kDa protein antibodyAdiponectin antibodyAdipoQ antibodyAdipose most abundant gene transcript 1 antibodyAdipose most abundant gene transcript 1 protein antibodyAdipose specific collagen like factor antibodyADIPQTL1 antibodyADPN antibodyAPM 1 antibodyapM-1 antibodyApM1 antibodyC1q and collagen domain-containing protein antibodyGBP 28 antibodyGBP28 antibodyGelatin binding protein antibodyGelatin binding protein 28 antibodyGelatin-binding protein antibody

  see all

Anti-Adiponectin antibody images

• 

  Western blot - Adiponectin antibody (ab59687)
  Anti-Adiponectin antibody (ab59687) at 1/2000 dilution + Mouse plasma at 1 µl
  
  Secondary
  Rabbit anti-IgY AP conjugate at 1/5000 dilution
  
  Predicted band size : 26 kDa
  Observed band size : 30 kDa (why is the actual band size different from the predicted?)