Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093)

Overview

  • Product name
    Anti-Adipose Triglyceride Lipase (phospho S406) antibody
    See all Adipose Triglyceride Lipase primary antibodies
  • Description
    Rabbit polyclonal to Adipose Triglyceride Lipase (phospho S406)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Dog, Pig, Chinese hamster
  • Immunogen

    Synthetic peptide within Mouse Adipose Triglyceride Lipase aa 400 to the C-terminus (phospho S406) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8BJ56
    (Peptide available as ab174058)

  • Positive control
    • This antibody gave a positive signal in both Mouse White Adipose Tissue from Gonard (High Fat Diet) and Mouse White Adipose Tissue from Subcutaneous & Visceral Fat - Fasted.

Properties

Applications

Our Abpromise guarantee covers the use of ab135093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.Can be blocked with Mouse Adipose Triglyceride Lipase (phospho S406) peptide (ab174058).

Target

  • Function
    Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
  • Tissue specificity
    Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).
  • Pathway
    Glycerolipid metabolism; triacylglycerol degradation.
  • Involvement in disease
    Note=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
    Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells.
  • Sequence similarities
    Contains 1 patatin domain.
  • Developmental stage
    Induced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium.
  • Cellular localization
    Lipid droplet. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110001C14Rik antibody
    • Adipose triglyceride lipase antibody
    • ATGL antibody
    • ATGL DESNUTRIN antibody
    • Calcium independent phospholipase A2 antibody
    • Calcium-independent phospholipase A2 antibody
    • Desnutrin antibody
    • EC 3.1.1.3 antibody
    • FP17548 antibody
    • IPLA2 zeta antibody
    • IPLA2-zeta antibody
    • Mutant patatin like phospholipase domain containing 2 antibody
    • Patatin like phospholipase domain containing 2 antibody
    • PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 antibody
    • Patatin-like phospholipase domain-containing protein 2 antibody
    • PEDF R antibody
    • PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA antibody
    • Pigment epithelium derived factor antibody
    • Pigment epithelium-derived factor antibody
    • plpl antibody
    • plpl2 antibody
    • PLPL2_HUMAN antibody
    • Pnpla2 antibody
    • Transport secretion protein 2 antibody
    • Transport secretion protein 2.2 antibody
    • Transport-secretion protein 2 antibody
    • Triglyceride hydrolase antibody
    • TTS 2.2 antibody
    • TTS2 antibody
    • TTS2.2 antibody
    • ZETA antibody
    see all

Images

  • All lanes : Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093) at 1 µg/ml

    Lane 1 : Mouse (Male) WAT from Gonard (High Fat Diet) Tissue Lysate
    Lane 2 : Mouse WAT (White Adipose Tissue) (Fasted) Tissue Lysate
    Lane 3 : Mouse (Male) WAT from Gonard (High Fat Diet) Tissue Lysate with Mouse Adipose Triglyceride Lipase (phospho S406) peptide (ab174058) at 1 µg/ml
    Lane 4 : Mouse WAT (White Adipose Tissue) (Fasted) Tissue Lysate with Mouse Adipose Triglyceride Lipase (phospho S406) peptide (ab174058) at 1 µg/ml
    Lane 5 : Mouse (Male) WAT from Gonard (High Fat Diet) Tissue Lysate with Mouse Adipose Triglyceride Lipase peptide (ab174059) at 1 µg/ml
    Lane 6 : Mouse WAT (White Adipose Tissue) (Fasted) Tissue Lysate with Mouse Adipose Triglyceride Lipase peptide (ab174059) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa


    Exposure time: 20 minutes
  • ab135093 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.

References

This product has been referenced in:
  • Iannucci LF  et al. Metabolomic analysis shows differential hepatic effects of T2 and T3 in rats after short-term feeding with high fat diet. Sci Rep 7:2023 (2017). Read more (PubMed: 28515456) »
  • Liu G  et al. ColXV promotes adipocyte differentiation via inhibiting DNA methylation and cAMP/PKA pathway in mice. Oncotarget 8:60135-60148 (2017). Read more (PubMed: 28947959) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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