The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
FunctionCa(2+)-regulated actin-binding protein. May have a unique function in the morphogenesis of neuronal cells which form ganglia. Required for SREC1-mediated regulation of neurite-like outgrowth. Plays a role in regenerative sensory axon outgrowth and remodeling processes after peripheral injury in neonates. Involved in the formation of long fine actin-containing filopodia-like structures in fibroblast. Plays a role in ciliogenesis.
Tissue specificityMost highly expressed in the small intestine and colonic lining. Weaker expression also detected in the thymus, prostate, testes and uterus.
Sequence similaritiesBelongs to the villin/gelsolin family. Contains 6 gelsolin-like repeats. Contains 1 HP (headpiece) domain.
All lanes : Anti-Advillin antibody (ab72210) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Mouse skin tissue lysate - total protein (ab4025)
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 92 kDa Observed band size : 92 kDa Additional bands at : 105 kDa,52 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Frozen sections) - Anti-Advillin antibody (ab72210)Image courtesy of Dr Xinpeng Dun by Abreview.
ab72210 staining Advillin in murine sciatic nerve tissue by Immunohistochemistry (Frozen sections). Tissue was fixed in formaldehyde, blocked with 3% BSA for 45 minutes and then incubated with ab72210 at a 1/500 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.