• Product nameAnti-Advillin antibody
    See all Advillin primary antibodies
  • Description
    Rabbit polyclonal to Advillin
  • Tested applicationsWB, IHC, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Mouse Advillin.

    (Peptide available as ab87446.)

  • Positive control
    • This antibody gave a positive signal in mouse brain and skin tissue lysates.



Our Abpromise guarantee covers the use of ab72210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).Can be blocked with Mouse Advillin peptide (ab87446).
IHC Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/500.


  • FunctionCa(2+)-regulated actin-binding protein. May have a unique function in the morphogenesis of neuronal cells which form ganglia. Required for SREC1-mediated regulation of neurite-like outgrowth. Plays a role in regenerative sensory axon outgrowth and remodeling processes after peripheral injury in neonates. Involved in the formation of long fine actin-containing filopodia-like structures in fibroblast. Plays a role in ciliogenesis.
  • Tissue specificityMost highly expressed in the small intestine and colonic lining. Weaker expression also detected in the thymus, prostate, testes and uterus.
  • Sequence similaritiesBelongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.
  • Cellular localizationCytoplasm > cytoskeleton. Cell projection. Cell projection > axon.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein DOC 6 antibody
    • Actin binding protein DOC6 antibody
    • Advil antibody
    • Advillin antibody
    • AVIL antibody
    • AVIL_HUMAN antibody
    • DOC 6 antibody
    • DOC6 antibody
    • p92 antibody
    see all

Anti-Advillin antibody images

  • All lanes : Anti-Advillin antibody (ab72210) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Skin (Mouse) Whole Cell Lysate - normal tissue (ab4025)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 92 kDa
    Observed band size : 92 kDa
    Additional bands at : 105 kDa,52 kDa. We are unsure as to the identity of these extra bands.
  • ab72210 staining Advillin in murine sciatic nerve tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in formaldehyde, blocked with 3% BSA for 45 minutes and then incubated with ab72210 at a 1/500 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

References for Anti-Advillin antibody (ab72210)

ab72210 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Sciatic nerve)
Specification Sciatic nerve
Fixative Formaldehyde
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3%

Dr. Xinpeng Dun

Verified customer

Submitted Jun 19 2012