Overview

  • Product nameAnti-Advillin antibodySee all Advillin primary antibodies ...
  • Description
    Rabbit polyclonal to Advillin
  • Tested applicationsWB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Mouse Advillin.

    (Peptide available as ab87446.)

  • Positive control
    • This antibody gave a positive signal in mouse brain and skin tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab72210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).Can be blocked with Mouse Advillin peptide (ab87446).
IHC-Fr 1/500.

Target

  • FunctionCa(2+)-regulated actin-binding protein. May have a unique function in the morphogenesis of neuronal cells which form ganglia. Required for SREC1-mediated regulation of neurite-like outgrowth. Plays a role in regenerative sensory axon outgrowth and remodeling processes after peripheral injury in neonates. Involved in the formation of long fine actin-containing filopodia-like structures in fibroblast. Plays a role in ciliogenesis.
  • Tissue specificityMost highly expressed in the small intestine and colonic lining. Weaker expression also detected in the thymus, prostate, testes and uterus.
  • Sequence similaritiesBelongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.
  • Cellular localizationCytoplasm > cytoskeleton. Cell projection. Cell projection > axon.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein DOC 6 antibody
    • Actin binding protein DOC6 antibody
    • Advil antibody
    • Advillin antibody
    • AVIL antibody
    • AVIL_HUMAN antibody
    • DOC 6 antibody
    • DOC6 antibody
    • p92 antibody
    see all

Anti-Advillin antibody images

  • All lanes : Anti-Advillin antibody (ab72210) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Skin (Mouse) Whole Cell Lysate - normal tissue (ab4025)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 92 kDa
    Observed band size : 92 kDa
    Additional bands at : 105 kDa,52 kDa. We are unsure as to the identity of these extra bands.
  • ab72210 staining Advillin in murine sciatic nerve tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in formaldehyde, blocked with 3% BSA for 45 minutes and then incubated with ab72210 at a 1/500 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

References for Anti-Advillin antibody (ab72210)

ab72210 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Sciatic nerve)
Specification Sciatic nerve
Fixative Formaldehyde
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3%
Username

Dr. Xinpeng Dun

Verified customer

Submitted Jun 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"