Overview
Product nameAeromomonas salmonicida OmpA protein (Active)
Protein descriptionRecombinant full length Aeromomonas salmonicida OmpA, 48kDa.
This protein comes from the gene for the monomeric form of OmpA from achromogenic atypical Aeromonas salmonicida.
N-terminal AAs not consistent with OmpA of Aeromonas salmonicida subsp. salmonicida A449.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab68485 was purified by proprietary chromatographic techniques.
Purity is greater than 98.0% as determined by analysis by RP-HPLC and SDS-PAGE .
FormLyophilised:Reconstitute in sterile 0.4% NaHCO3.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferThe protein was lyophylized in presence of NaHCO3 at 2:1 protein:salt ratio.
Concentration information loading...
Sequence notesThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Val-Val-Ile-Ser.
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab68485
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| Functional Studies |
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| SDS-PAGE |
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Application notesBiological Activity: Use at an assay dependent dilution.
Thioglycolate elicited peritoneal macrophages exhibited a low level of activation (18% cytotoxicity) that was significantly increased (48% cytotoxicity) in the presence of latex beads. Coating of the latex beads with this protein resulted in an increase of cytoxicity (mean +/- SEM) from 48% to 91%.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
RelevanceAeromonas salmonicida is the causative agent of goldfish ulcer disease. Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein also known as OmpA.
This protein comes from the gene for the monomeric form of OmpA from achromogenic atypical Aeromonas salmonicida.
References for Aeromomonas salmonicida OmpA protein (Active) (ab68485)
ab68485
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
