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Products:Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Other gram-ve bacteria
Overview
Product name
Aeromomonas salmonicida OmpA protein (Active)
Protein description
Recombinant full length Aeromomonas salmonicida OmpA, 48kDa. This protein comes from the gene for the monomeric form of OmpA from achromogenic atypical Aeromonas salmonicida. N-terminal AAs not consistent with OmpA of Aeromonas salmonicida subsp. salmonicida A449.
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Purification notes
ab68485 was purified by proprietary chromatographic techniques. Purity is greater than 98.0% as determined by analysis by RP-HPLC and SDS-PAGE .
Form
Lyophilised:
Reconstitute in sterile 0.4% NaHCO3.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
The protein was lyophylized in presence of NaHCO3 at 2:1 protein:salt ratio.
Concentration
Concentration information loading...
Sequence notes
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Va l-Val-Ile- Ser.
Research areas
Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Other gram-ve bacteria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Functional Studies
SDS-PAGE
Application notes
Biological Activity: Use at an assay dependent dilution.
Thioglycolate elicited peritoneal macrophages exhibited a low level of activation (18% cytotoxicity) that was significantly increased (48% cytotoxicity) in the presence of latex beads. Coating of the latex beads with this protein resulted in an increase of cytoxicity (mean +/- SEM) from 48% to 91%.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Relevance
Aeromonas salmonicida is the causative agent of goldfish ulcer disease. Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein also known as OmpA. This protein comes from the gene for the monomeric form of OmpA from achromogenic atypical Aeromonas salmonicida.
References for Aeromomonas salmonicida OmpA protein (Active) (ab68485)
ab68485 has not yet been referenced specifically in any publications.
Publishing research using ab68485? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"