Aggrecan ARGxx antibody [BC-3] (ab3773)

Product Name 

Aggrecan ARGxx antibody [BC-3]

Product type 

Primary antibodies

Description 

Mouse monoclonal [BC-3] to Aggrecan ARGxx

Immunogen 

ARGSV... synthetic peptide conjugate.
BLAST 'ARGSV' with or

Reacts with 

Hu, Rat, Cat, Cow, Dog, Gpig, Hrs, Pig, Rb, Shp

Does not react with 
(species key)

Ms

Specificity 

Recognises the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration).
This antibody will not recognise the sequence ...ARG.. if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognise the aggrecanase generated neoepitope ARG...

Samples need to be deglycosylated for epitope recognition (see notes).

Tested applications 
(see key)

ELISA, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependant dilution.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
WB: 1/100. Detects a variety of epitopes between 50 and 250 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections.

Cellular localization 

Secreted

Research areas 

Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Aggrecan
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker

Relevance 

Aggrecan has been detected in neural precursor cells (neurospheres; Kabos et al, 2004) During differentiation, neurospheres downregulate Chondroitin sulfate proteoglycans (CSPGs). Proliferating neural precursors synthesize lecticans, including aggrecan, which are downregulated with differentiation; suggesting a link between CSPGs and CNS precursor biology.

Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

BC-3

Myeloma 

x63-Ag8.653

Isotype 

IgG1

Light chain type 

kappa

Purity 

Tissue culture supernatant

Storage buffer 

0.2M Tris/HCl pH 7.4, 1-10% foetal calf serum, 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al.

Diluted Technomouse culture supernatant.

References for Aggrecan ARGxx antibody [BC-3] (ab3773)

  This product has been used in: (two most recent references)

Echtermeyer F et al. Syndecan-4 regulates ADAMTS-5 activation and cartilage breakdown in osteoarthritis. Nat Med 15:1072-6 (2009). IHC-P. PubMed: 19684582

Rousseau JC et al. Patients with rheumatoid arthritis have an altered circulatory aggrecan profile. BMC Musculoskelet Disord 9:74 (2008). WB; Human. PubMed: 18507823

See all 19 publication references for this product.


If you publish research using ab3773 please let us know so that we can cite the reference on this datasheet.

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