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Anti-Aggrecan ARGxx antibody [BC-3] (ab3773)

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Overview

Product name

Anti-Aggrecan ARGxx antibody [BC-3]

Description

Mouse monoclonal [BC-3] to Aggrecan ARGxx

Specificity

Recognises the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration). This antibody will not recognise the sequence ...ARG.. if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognise the aggrecanase generated neoepitope ARG... Samples need to be deglycosylated for epitope recognition (see notes).

Tested applications

ELISA, IHC-P, IHC-Fr, WB, Sandwich ELISAmore details

Cross reactivity

Reacts with

Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig

Does not react with

Mouse

Immunogen

ARGSV... synthetic peptide conjugate.

ARGSV

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

General notes

Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. Diluted Technomouse culture supernatant.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

0.2M Tris/HCl pH 7.4, 1-10% foetal calf serum, 0.02% sodium azide

Purity

Tissue culture supernatant

Primary antibody notes

Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.

Clonality

Monoclonal

Clone number

BC-3

Myeloma

x63-Ag8.653

Isotype

IgG1

Light chain type

kappa

Research areas

Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Aggrecan
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependant dilution.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
sELISA: Use at an assay dependent dilution (PMID 19932675).
WB: 1/100. Detects a variety of epitopes between 50 and 250 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections.

Target

Relevance

Aggrecan has been detected in neural precursor cells (neurospheres; Kabos et al, 2004) During differentiation, neurospheres downregulate Chondroitin sulfate proteoglycans (CSPGs). Proliferating neural precursors synthesize lecticans, including aggrecan, which are downregulated with differentiation; suggesting a link between CSPGs and CNS precursor biology.

Cellular localization

Secreted

Alternative names

  • AGC 1 antibody
  • AGC1 antibody
  • AGC1 protein antibody
  • Aggrecan 1 antibody
  • Aggrecan core protein antibody
  • Aggrecan1 antibody
  • Antigen identified by monoclonal antibody A0122 antibody
  • Cartilage specific proteoglycan core protein antibody
  • Chondroitin sulfate proteoglycan 1 antibody
  • Chondroitin sulfate proteoglycan core protein 1 antibody
  • CSPCP antibody
  • CSPG 1 antibody
  • CSPG1 antibody
  • CSPGCP antibody
  • Large aggregating proteoglycan antibody
  • MSK16 antibody
  • SEDK antibody
see all

Database links

see all

Protocols

  • Recommended WB protocol for ab3773
  • Recommended IHC-P protocol with ab3773

    • References for Anti-Aggrecan ARGxx antibody [BC-3] (ab3773)

    This product has been referenced in:

    • Wang Jet al. TNF-a and IL-1ß Promote a Disintegrin-like and Metalloprotease with Thrombospondin Type I Motif-5-mediated Aggrecan Degradation through Syndecan-4 in Intervertebral Disc. J Biol Chem 286:39738-49 (2011). WB; Human.Read more (PubMed: 21949132) »
    • Torzilli PAet al. Mechanical load inhibits IL-1 induced matrix degradation in articular cartilage. Osteoarthritis Cartilage 18:97-105 (2010).Read more (PubMed: 19747586) »

    See all 12 publications for this product

    Publishing research using ab3773? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"