Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
|
ab32380 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab32381? Please let us know so that we can cite the reference in this datasheet
ab32381 has been referenced in 7 publications.
Publishing research using ab32381? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Ago2 / eIF2C2 antibody - ChIP Grade (ab32381) at 2 µg/ml The identification of the 55 kDa band is unclear but this band has also been observed in Hela lysates in the following publication; http://www.ibiosci.or.kr/home/data/2004/0080213.pdf and in the Western Blot of ab5072, targeting Drosophila Ago2 / eIF2C2.
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 97 kDa
Observed band size : 87 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
IHC image of Ago2/eIF2C2 staining in human breast cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32381, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab32381 (1/200) staining Ago2/ eIF2C2 in assynchrnous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% TritonX100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
6
Call 01223 696 000 or contact us