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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Overview
Product name
Anti-Ago2 / eIF2C2 antibody - ChIP Grade
See all Ago2 / eIF2C2 products (3) ...
Description
Mouse monoclonal to Ago2 / eIF2C2 - ChIP Grade
Tested applications
WB, ICC/IF, IHC-P, IP, Flow Cyt, ChIPmore details
Cross reactivity
Reacts with
Chicken, Human, Zebrafish
Immunogen
Recombinant full length protein, corresponding to amino acids 1-377 of Human Ago2 / eIF2C2
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Applications
Show applications key
Our Abpromise guarantee covers the use of ab57113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 97 kDa.
ICC/IF: Use a concentration of 10 µg/ml
IHC-P: Use a concentration of 3 µg/ml
IP: Use at an assay dependent dilution. (PubMed: 19574298)
Flow Cyt: Use 1µg for 106 cells.
ChIP: Use at an assay dependent dilution. (PubMed: 20010811)
Target
Function
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
Domain
The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH).
Post-translational
modifications
Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity.
Cellular localization
Cytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.
Target information above from: UniProt accessionQ9UKV8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ago 2 antibody
- AGO2_HUMAN antibody
- Argonaute 2 antibody
see all
Database links
- Entrez Gene: 27161 Human
- SwissProt: Q9UKV8 Human
- Unigene: 449415 Human
Anti-Ago2 / eIF2C2 antibody - ChIP Grade images:
Immunocytochemistry/ Immunofluorescence - Ago2 / eIF2C2 antibody - ChIP Grade (ab57113)
Ago2 / eIF2C2 antibody (ab57113) used in immunofluorescence at 10ug/ml on HeLa cells.
IHC-P - Ago2 / eIF2C2 antibody (ab57113)
Ago2 / eIF2C2 antibody (ab57113) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.
- Ago2 / eIF2C2 antibody (ab57113)
Western Blot detection against the recombinant fragment imunogen (68.4 KDa).
Flow Cytometry-Ago2 / eIF2C2 antibody - ChIP Grade(ab57113)
Overlay histogram showing HeLa cells stained with ab57113 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57113, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-Ago2 / eIF2C2 antibody - ChIP Grade (ab57113)
This product has been referenced in:
- Behrman Set al. A CHOP-regulated microRNA controls rhodopsin expression. J Cell Biol 192:919-27 (2011). WB; Human.Read more (PubMed: 21402790) »
- Joyce CEet al. Deep sequencing of small RNAs from human skin reveals major alterations in the psoriasis miRNAome. Hum Mol Genet 20:4025-40 (2011). WB.Read more (PubMed: 21807764) »
See all 10 publications for this product
Publishing research using ab57113? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"