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Western blot - Ago2 / eIF2C2 antibody (ab32381)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ago2 / eIF2C2 antibody (ab32381)
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Immunocytochemistry/ Immunofluorescence - Ago2 / eIF2C2 antibody (ab32381)
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Product Name
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Ago2 / eIF2C2 antibody
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See all Ago2 / eIF2C2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Ago2 / eIF2C2
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 - 450 Ago2 / eIF2C2. (Peptide available as ab32380.)
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Reacts with
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Hu, Ms
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Does not react with
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Dm
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Predicted to react
(species key)
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Rat, Cow, Rb
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/200.
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 97 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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ab32381 gives a positive result in Western Blot in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line),
Jurkat (Human T cell lymphoblast-like cell line),
HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
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Cellular localization
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Cytoplasmic (P-bodies)
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
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Relevance
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Ago2 is essential for RNA interference (RNAi), where double-stranded RNA induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys messenger RNAs homologous to the silencing trigger.
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Ago2 / eIF2C2 antibody (ab32381) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.90 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Ago2 / eIF2C2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Ago2 / eIF2C2 antibody images, references, products related to ab32381 and other tools.
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Ago2 / eIF2C2 antibody images:
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 Western blot - Ago2 / eIF2C2 antibody (ab32381)
All lanes : Ago2 / eIF2C2 antibody (ab32381) at 2 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 97 kDa
Observed band size : 87 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
The identification of the 55 kDa band is unclear but this band has also been observed in Hela lysates in the following publication; http://www.ibiosci.or.kr/home/data/2004/0080213.pdf and in the Western Blot of ab5072, targeting Drosophila Ago2 / eIF2C2.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ago2 / eIF2C2 antibody (ab32381)
IHC image of Ago2/eIF2C2 staining in human breast cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32381, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunocytochemistry/ Immunofluorescence - Ago2 / eIF2C2 antibody (ab32381)
ab32381 (1/200) staining Ago2/ eIF2C2 in assynchrnous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% TritonX100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details. This image is courtesy of an Abreview submitted by Dr Kirk McManus
See Abreview
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References for Ago2 / eIF2C2 antibody (ab32381)
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This product has been used in: (two most recent references)
Chen TS et al. Mesenchymal stem cell secretes microparticles enriched in pre-microRNAs. Nucleic Acids Res 38:215-24 (2010). WB; Human. PubMed: 19850715
Turunen MP et al. Efficient regulation of VEGF expression by promoter-targeted lentiviral shRNAs based on epigenetic mechanism: a novel example of epigenetherapy. Circ Res 105:604-9 (2009). ChIP. PubMed: 19696410
See all 3 publication references for this product.
If you publish research using ab32381 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Ago2 / eIF2C2
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Ago2 / eIF2C2 antibody - more information
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