Ago2 / eIF2C2 antibody (ab5072)

Product Name 

Ago2 / eIF2C2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Ago2 / eIF2C2

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Fruit fly (Drosophila melanogaster) Ago2.

(Peptide available as ab24177.)

Reacts with 

Dm

Does not react with 
(species key)

Hu

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 55 + 95 kDa (predicted molecular weight: 106 kDa). Swissprot suggests Ago 2 may have 50kDa form.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic (P-bodies)

Research areas 

Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> RNAi >> Eukaryotic Initiation factors (eIF's)
Chromatin and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

Relevance 

Ago2 is essential for RNA interference (RNAi), where double-stranded RNA induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys messenger RNAs homologous to the silencing trigger.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Please note the immunogen for this antibody has no sequence alignment to human dIFC2. When tested in human, non-specific binding is observed.

See below for Ago2 / eIF2C2 antibody images, references, products related to ab5072 and other tools.

Ago2 / eIF2C2 antibody images:

References for Ago2 / eIF2C2 antibody (ab5072)

  This product has been used in:

Schneider MD et al. Gawky is a component of cytoplasmic mRNA processing bodies required for early Drosophila development. J Cell Biol 174:349-58 (2006). PubMed: 16880270
If you publish research using ab5072 please let us know so that we can cite the reference on this datasheet.

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