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Products:Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Overview
Product name
Anti-Agpat2 antibody
See all Agpat2 products (3) ...
Description
Mouse polyclonal to Agpat2
Tested applications
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human
Immunogen
Fusion protein: LLRHGGRTVDNMSIISWFVR, corresponding to amino acids 49/68 of Mouse Agpat2.
LLRHGGRTVD NMSIISWFVR
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
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Western blot - Agpat2 antibody (ab21816)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 31 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Function
Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
Tissue specificity
Expressed predominantly in heart and liver.
Pathway
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Involvement in disease
Defects in AGPAT2 are the cause of congenital generalized lipodystrophy type 1 (CGL1) [MIM:608594]; also known as Berardinelli-Seip congenital lipodystrophy type 1 (BSCL1) or Berardinelli-Seip syndrome. CGL1 is an autosomal recessive disorder characterized by marked paucity of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
Sequence similarities
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
Cellular localization
Membrane.
Target information above from: UniProt accessionO15120
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1 acyl sn glycerol 3 phosphate acyltransferase beta antibody
- 1 acylglycerol 3 phosphate O acyltransferase 2 antibody
- 1 AGP acyltransferase 2 antibody
see all
Database links
- Entrez Gene: 10555 Human
- Entrez Gene: 67512 Mouse
- Omim: 603100 Human
- SwissProt: O15120 Human
- SwissProt: Q8K3K7 Mouse
- Unigene: 320151 Human
- Unigene: 24244 Mouse
Anti-Agpat2 antibody images:
Western blot - Agpat2 antibody (ab21816)
All lanes : Anti-Agpat2 antibody (ab21816) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 31 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
References for Anti-Agpat2 antibody (ab21816)
ab21816 has not yet been referenced specifically in any publications.
Publishing research using ab21816? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"