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Western blot - Agpat2 antibody (ab21816)
(enlarge)
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Product Name
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Agpat2 antibody
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See all Agpat2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to Agpat2
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Immunogen
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Fusion protein: LLRHGGRTVDNMSIISWFVR, corresponding to amino acids 49/68 of Mouse Agpat2.
BLAST 'LLRHGGRTVDNMSIISWFVR' with  or 
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Reacts with
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Ms
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Predicted to react
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Hu
(Due to sequence homology)
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 31 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
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Cellular localization
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Membrane; Multi-pass membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
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Relevance
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Agpat2 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. It is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in its have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Constituents: 50% Glycerol Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Notes
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Produced from outbred CD1 mice
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Agpat2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Agpat2 antibody images, references, products related to ab21816 and other tools.
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Agpat2 antibody images:
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 Western blot - Agpat2 antibody (ab21816)
All lanes : Agpat2 antibody (ab21816) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 31 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
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Search PubMed (MEDLINE) for references to Agpat2
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Agpat2 antibody - more information
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