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Western blot - Agpat2 antibody (ab21816)
Western blot - Agpat2 antibody (ab21816)
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Product Name 

Agpat2 antibody

 

See all Agpat2 antibodies (2)...

Product type 

Primary antibodies

Description 

Mouse polyclonal to Agpat2

Immunogen 

Fusion protein: LLRHGGRTVDNMSIISWFVR, corresponding to amino acids 49/68 of Mouse Agpat2.
BLAST 'LLRHGGRTVDNMSIISWFVR' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Ms

Predicted to react 
(species key)

Hu
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 31 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Cellular localization 

Membrane; Multi-pass membrane protein.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis

Relevance 

Agpat2 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. It is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in its have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.


This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.

A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.

Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

 

Alternative names Agpat2 antibody (ab21816)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

10555    (Human)
67512    (Mouse)

Omim

    

603100    (Human)

SwissProt

    

O15120    (Human)
Q8K3K7    (Mouse)

Unigene

    

320151    (Human)

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Constituents: 50% Glycerol

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Notes 

Produced from outbred CD1 mice

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Agpat2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Agpat2 antibody images, references, products related to ab21816 and other tools.

 

Agpat2 antibody images:

Product image  Western blot - Agpat2 antibody (ab21816)

Western blot - Agpat2 antibody (ab21816)

All lanes : Agpat2 antibody (ab21816) at 1/1000 dilution

Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

Predicted band size : 31 kDa


The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.

 
 

Search PubMed (MEDLINE) for references to Agpat2

 

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