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Western blot - Agpat2 antibody (ab62599)
(enlarge)
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Product Name
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Agpat2 antibody
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See all Agpat2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Agpat2
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Immunogen
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A region within synthetic peptide: IPTSGLTAAD VPALVDTCHR AMRTTFLHIS KTPQENGATA GSGVQPAQ, corresponding to C terminal amino acids 199-246 of Human Agpat2
BLAST 'IPTSGLTAAD VPALVDTCHR AMRTTFLHIS KTPQENGATA ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1/312500.
WB: Use at a concentration of 5 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate.
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Cellular localization
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Membrane; Multi-pass membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
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Relevance
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Agpat2 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. It is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in its have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Agpat2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Agpat2 antibody images, references, products related to ab62599 and other tools.
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Agpat2 antibody images:
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 Western blot - Agpat2 antibody (ab62599)
Lane 1 : Molecular weight marker
Lane 2 : Agpat2 antibody (ab62599) at 5 µg/ml
Lane 1 : As above
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 31 kDa
Observed band size : 31 kDa
Gel concentration 12%
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Search PubMed (MEDLINE) for references to Agpat2
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Agpat2 antibody - more information
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Customer reviews (feedback) regarding Agpat2 antibody |
Customer FAQs regarding Agpat2 antibody |
Protocols for Agpat2 antibody |
Price and availability of products related to Agpat2 antibody |
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Agpat2 antibody - related products:
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Agpat2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Agpat2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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