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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Overview
Product name
Anti-Agrin antibody [AGR 131]
See all Agrin products (3) ...
Description
Mouse monoclonal [AGR 131] to Agrin
Specificity
Ab12362 detects all isoforms of agrin. This antibody recognizes all N1 isoforms but, consistent with the epitope mapping investigations, fails to see the N4 protein. Furthermore, the antibody will detect the X, Y and Z splice variants.
Tested applications
Cross reactivity
Reacts with
Rat
Immunogen
Recombinant rat agrin, C-terminal construct.
Epitope
Epitope is located near the 1st EGF-like repeat.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
AGR 131
Isotype
IgG1
Research areas
Neuroscience >> Neurology process >> Neural Signal Transduction
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Applications
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Our Abpromise guarantee covers the use of ab12362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1000 µg/mlPredicted molecular weight: 208.6 kDa.
Target
Relevance
Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
Cellular localization
Secreted; extracellular space; extracellular matrix. Note: Synaptic basal lamina at the neuromuscular junction.
Alternative names
- AGRIN antibody
- Agrin proteoglycan antibody
- AGRN antibody
see all
Database links
- Entrez Gene: 25592 Rat
- SwissProt: P25304 Rat
- Unigene: 2163 Rat
References for Anti-Agrin antibody [AGR 131] (ab12362)
ab12362 has not yet been referenced specifically in any publications.
Publishing research using ab12362? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"