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Product name
Anti-Agrin antibody [Agr 247]
See all Agrin products (3) ...
Description
Mouse monoclonal [Agr 247] to Agrin
Specificity
Ab12364 regognises agrin.
Tested applications
ICCmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Recombinant rat agrin, C-terminal construct.
Epitope
Ab12364 binds to an epitope close to splicing site Z and causes a marked reduction in the number of nicotinic acetylcholine receptor(nAChR) clusters in samples from mouse and rat origins.
Positive control
Mouse Brain Tissue Extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
Agr 247
Isotype
IgG1
Research areas
Neuroscience >> Neurology process >> Neural Signal Transduction
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use a concentration of 2 µg/ml.
Target
Relevance
Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
Cellular localization
Secreted; extracellular space; extracellular matrix. Note: Synaptic basal lamina at the neuromuscular junction.
Alternative names
- AGRIN antibody
- Agrin proteoglycan antibody
- AGRN antibody
see all
Database links
- Entrez Gene: 11603 Mouse
- Entrez Gene: 25592 Rat
- SwissProt: A2ASQ1 Mouse
- SwissProt: P25304 Rat
- Unigene: 2163 Rat
References for Anti-Agrin antibody [Agr 247] (ab12364)
This product has been referenced in:
- Tourovskaia A et al. Local induction of acetylcholine receptor clustering in myotube cultures using microfluidic application of agrin. Biophys J 90:2192-8 (2006).Read more (PubMed: 16387765) »
See 1 publication for this product
Publishing research using ab12364? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"