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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Overview
Product name
Anti-Agrin antibody
See all Agrin products (3) ...
Description
Rabbit polyclonal to Agrin
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide from the N terminal residues of Human Agrin protein (NM_198576).
Positive control
Human skeletal muscle.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, 0.1M Tris glycine, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neural Signal Transduction
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Agrin antibody (ab85174)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab85174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-P: 1/100 - 1/500.
Peptide ELISA titer: 1/10000 - 1/40000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
Cellular localization
Secreted; extracellular space; extracellular matrix. Note: Synaptic basal lamina at the neuromuscular junction.
Alternative names
- AGRIN antibody
- Agrin proteoglycan antibody
- AGRN antibody
see all
Database links
- Entrez Gene: 375790 Human
- Entrez Gene: 11603 Mouse
- Entrez Gene: 25592 Rat
- Omim: 103320 Human
- SwissProt: O00468 Human
- SwissProt: A2ASQ1 Mouse
- SwissProt: P25304 Rat
- Unigene: 273330 Human
see all
Anti-Agrin antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Agrin antibody (ab85174)
ab85174, at 1/100 dilution, staining Agrin in formalin-fixed, paraffin-embedded human skeletal muscle by Immunohistochemistry.
References for Anti-Agrin antibody (ab85174)
ab85174 has not yet been referenced specifically in any publications.
Publishing research using ab85174? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"