Recombinant Anti-Ahsp antibody [EPR12319] (ab180861)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12319] to Ahsp
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Ahsp antibody [EPR12319]
See all Ahsp primary antibodies -
Description
Rabbit monoclonal [EPR12319] to Ahsp -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver lysate; Human fetal spleen tissue, Human lung tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12319 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 12 kDa.
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IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 12 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
Target
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Function
Act as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. -
Tissue specificity
Expressed in blood and bone marrow. -
Sequence similarities
Belongs to the AHSP family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 51327 Human
- Omim: 605821 Human
- SwissProt: Q9NZD4 Human
- Unigene: 274309 Human
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Alternative names
- AHSP antibody
- AHSP_HUMAN antibody
- Alpha hemoglobin stabilizing protein antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Ahsp with ab180861 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Anti-Ahsp antibody [EPR12319] (ab180861) at 1/1000 dilution + Human fetal liver tissue lysate at 10 µg
Predicted band size: 12 kDa -
Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab180861 at 1/10 dilution.
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Immunohistochemical analysis of paraffin-embedded Human fetal spleen tissue labeling Ahsp with ab180861 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab180861 has not yet been referenced specifically in any publications.