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Western blot - Ajuba antibody (ab67666)
(enlarge)
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Product Name
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Ajuba antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to Ajuba
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Immunogen
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Full length Ajuba protein (Human)
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is reactive against mammalian transfected lysate.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Ajuba transfected 293T cell lysate
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Cellular localization
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Cytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Centrosome.
Note: Shuttles between the cytoplasm and the nucleus.
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Research areas
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Stem Cells >> Signaling Pathways >> Wnt >> Nuclear
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial
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Relevance
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Ajuba is a member of LIM domain containing proteins that contribute to cell fate determination and regulate cell proliferation and differentiation. Ajuba is also involved in the regulation of actin cytoskeleton dynamics and cell migration. Arjuba plays a important role in the regulation of the kinase activity of AURKA/Aurora A for mitotic commitment. Is also a component of the IL 1 signaling pathway modulating IL 1 induced NF kappa B activation by influencing the assembly and activity of the PRKCZ/SQSTM1/TRAF6 multiprotein signaling complex. Ajuba is also a negative regulator of the Wnt signaling pathway.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Ajuba antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Ajuba antibody images, references, products related to ab67666 and other tools.
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Ajuba antibody images:
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 Western blot - Ajuba antibody (ab67666)
All lanes : Ajuba antibody (ab67666) at 1/500 dilution
Lane 1 : Ajuba transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 57 kDa
Observed band size : 57 kDa
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Search PubMed (MEDLINE) for references to Ajuba
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Ajuba antibody - more information
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Customer reviews (feedback) regarding Ajuba antibody |
Customer FAQs regarding Ajuba antibody |
Protocols for Ajuba antibody |
Price and availability of products related to Ajuba antibody |
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Ajuba antibody - related products:
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Ajuba antibody - other tools:
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Contact Abcam with a Technical Enquiry about Ajuba antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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