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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial
Overview
Product name
Anti-Ajuba antibody
See all Ajuba products (2) ...
Description
Mouse polyclonal to Ajuba
Specificity
This antibody is reactive against mammalian transfected lysate.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length Ajuba protein (Human)
Positive control
Ajuba transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Nuclear
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial
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Western blot - Ajuba antibody (ab67666)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Ajuba is a member of LIM domain containing proteins that contribute to cell fate determination and regulate cell proliferation and differentiation. Ajuba is also involved in the regulation of actin cytoskeleton dynamics and cell migration. Arjuba plays a important role in the regulation of the kinase activity of AURKA/Aurora A for mitotic commitment. Is also a component of the IL 1 signaling pathway modulating IL 1 induced NF kappa B activation by influencing the assembly and activity of the PRKCZ/SQSTM1/TRAF6 multiprotein signaling complex. Ajuba is also a negative regulator of the Wnt signaling pathway.
Cellular localization
Cytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Centrosome. Note: Shuttles between the cytoplasm and the nucleus.
Database links
- Entrez Gene: 84962 Human
- Omim: 609066 Human
- SwissProt: Q96IF1 Human
- Unigene: 434997 Human
Anti-Ajuba antibody images:
Western blot - Ajuba antibody (ab67666)
All lanes : Anti-Ajuba antibody (ab67666) at 1/500 dilution
Lane 1 : Ajuba transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 57 kDa
Observed band size : 57 kDa
References for Anti-Ajuba antibody (ab67666)
ab67666 has not yet been referenced specifically in any publications.
Publishing research using ab67666? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"