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Akt Kinase Activity Assay Kit (ab139436) is a non-radioactive assay providing a safe, rapid and reliable method for the screening of inhibitors or activators of Akt and for quantitating the activity of Akt in purified or partially purified enzyme preparations. This kit is based on a solid phase enzyme-linked immuno-absorbent assay (ELISA) that utilizes a specific synthetic peptide as a substrate for Akt and a polyclonal antibody that recognizes the phosphorylated form of the substrate. The assay is designed for the analysis of Akt activity in the solution phase. For the measurement of Akt in partially purified and purified enzyme preparations from any species.
The kit offers the following advantages:
1. Safe - non-radioactive measurement of kinase activity
2. Flexible - kinetic and end-point options available
3. Fast - results in < 4.5 hours
4. Efficient - only 30 µl diluted sample needed per well
|Components||1 x 96 tests|
|20X Wash Buffer||1 x 30ml|
|Active Akt||1 x 28µl|
|Akt Phosphospecific Substrate Antibody||1 x 5ml|
|Akt Substrate Microtiter Plate||1 unit|
|Antibody Dilution Buffer||1 x 10ml|
|Anti-Rabbit IgG: HRP Conjugate||1 x 20µl|
|ATP||1 x 2mg|
|Kinase Assay Dilution Buffer||1 x 10ml|
|Stop Solution 2||1 x 10ml|
|TMB Substrate||1 x 10ml|
Our Abpromise guarantee covers the use of ab139436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Varying quantities of purified active Akt were assayed using the Akt substrate microtiter plate. Assays were incubated for 60 minutes at 30°C.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"