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Alanine Transaminase Activity Assay Kit (ab105134)

Submit an Abreview Q&A (16)

Overview

  • Product nameAlanine Transaminase Activity Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 10 mU/well
  • Product overview

    In Abcam's Alanine Transaminase Activity Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ?-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Alanine transaminase (ALAT) is a transaminase enzyme (EC 2.6.1.2). It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALT). ALT is found in serum and in various bodily tissues, but is usually associated with the liver. It catalyzes the reaction: α-ketoglutarate + alanine ? glutamate + pyruvate. It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L).

  • Tested applicationsFunctional Studies more details

Properties

Applications

Our Abpromise guarantee covers the use of ab105134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies FuncS

Alanine Transaminase Activity Assay Kit images

  • Functional Studies - Alanine Transaminase Activity Assay Kit (ab105134)
    Functional Studies - Alanine Transaminase Activity Assay Kit (ab105134)
    Examples of Standard Curve and Positive Control analyses obtained with ab105134

Protocols

References for Alanine Transaminase Activity Assay Kit (ab105134)

ab105134 has not yet been referenced specifically in any publications.

Publishing research using ab105134 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab105134 Submit a Question for ab105134

Displaying 1 - 10 of 16 results for Abreviews and Q&A

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question concernant kit ab105134.

J’ai eu une réponse du laboratoire qui m’a confirmé que ce kit peut être utilisé sur des échantillons humain.

Malheureusement le laboratoire n’a pas de conversion entre l’activité ...

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Hallo!
Meine Frage bezieht sich auf Ihre Produkte
Alanine Transaminase Activity Assay Kit (ab105134)
und GST Activity Assay Kit (Colorimetric) (ab65326).
Ich möchte diese Kits nutzen, um die beiden Enzyme im Überstand meiner ...

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Ich möchte hiermit auch bestätigen, dass beide Kits für Zellkulturüberstand getestet und auch garantiert sind.

Sie haben Recht, dass eine Probenaufbereitung nicht gesondert erwähnt wird.

Zellkultur&uu...

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Dear,

Thank you for getting back to me - before we go any further to ordering, I would like a quote on the ALT positive control..
Hope to hear from you soon

I am sorry for the delay in getting back to you. I now have some further information to share with you.

Contrary to what I mentioned in my previous email, the L-Alanine Assay Kit (ab83394) is unlikely to be of use in the experiments you are perfo...

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I have another question regarding this kit, if the standard reaction does not include the ALT enzyme, is the positive control supplied in this kit is the ALT enzyme, if so, then we may be able to include this enzyme in the reaction in place of t...

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Thank you for contacting us yesterday in regards to the Alanine Transaminase Activity Assay Kit (ab105134). I have now had a chance to have a look at this product to see if it would be suitable to use in your experiment to assess the transaminase inhibiti...

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We purchased 2 kits ab105134.
Backfround is too high to obtain an accurate reading from positive control. in both kits. Second kit is worse than the first.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this kit is tested and covered by our 6 month guarantee for. In the eve...

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Dear,



I have a question about the assaykit Alanine transaminase activity kit number: ab105134



The section measurement of the protocol is a bit unclear to me. I would like to know whether after pipeting all the...

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Thank you for contacting us.

You are correct, the first readin should be done at RT after 10 minutes. After this you can incubate at 37 degrees away from light before taking the second reading. We have not tested this product using RO water and...

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Customer kindly contacted us as they were experiencing troubles with this product.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ...

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Can I use whole blood with this kit?

Thank you for your inquiry.

I heard back from the lab that whole blood cannot be used with this assay kit as the color will disrupt the reading. You will have to get plasma/serum to use it.

I hope this information helps. Please contact...

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We may not be able to purchase the kit prior to sampling (next week). We were hoping to place the tissue (liver) in ice cold SEI Buffer (150 mM sucrose, 10 mM EDTA, and 50 mM Imidazole), allow the buffer perfuse on ice for 30 minutes, then freeze in liqui...

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The consensus is that your sample prep and storage should be fine, though we do not have activity data for this enzyme for similar storage conditions.

Please let me know if you have any other questions or concerns.

What is the appropriate storage condition for tissue samples to be assayed for alanine transaminase?

Thank you for contacting us.

The developer of the kit tells us that samples can be stored frozen in the assay buffer that is included with the kit (25 ml). I think his assumption is that you will prepare homogenates of the tissue in the buffer ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"