Alanine Transaminase Activity Assay Kit (ab105134)
- Product nameAlanine Transaminase Activity Assay Kit
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
- Assay typeQuantitative
- Sensitivity> 10 mU/well
- Product overview
In Abcam's Alanine Transaminase Activity Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ?-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.
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Alanine transaminase (ALAT) is a transaminase enzyme (EC 220.127.116.11). It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALT). ALT is found in serum and in various bodily tissues, but is usually associated with the liver. It catalyzes the reaction: α-ketoglutarate + alanine ? glutamate + pyruvate. It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L).
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests ALT Positive Control (lyophilized) Blue 1 vial ALT Substrate (lyophilized) Orange 1 vial Pyruvate Standard (100 nmol/µl) Yellow 1 x 100µl ALT Assay Buffer WM 1 x 25ml ALT Enzyme Mix (lyophilized) Green 1 vial OxiRed™ (in DMSO Red 1 x 200µl
- Research Areas
Our Abpromise guarantee covers the use of ab105134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Alanine Transaminase Activity Assay Kit images
Examples of Standard Curve and Positive Control analyses obtained with ab105134
References for Alanine Transaminase Activity Assay Kit (ab105134)
ab105134 has not yet been referenced specifically in any publications.