Anti-Alanine Transaminase antibody (ab137531)
Key features and details
- Rabbit polyclonal to Alanine Transaminase
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Alanine Transaminase antibody
See all Alanine Transaminase primary antibodies -
Description
Rabbit polyclonal to Alanine Transaminase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 6-188 of Human Alanine Transaminase (NP_005300).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt4, Raji, mouse liver whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
Target
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Function
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. -
Tissue specificity
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue. -
Pathway
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 539188 Cow
- Entrez Gene: 2875 Human
- Entrez Gene: 76282 Mouse
- Entrez Gene: 81670 Rat
- Omim: 138200 Human
- SwissProt: A4IFH5 Cow
- SwissProt: P24298 Human
- SwissProt: Q8QZR5 Mouse
see all -
Alternative names
- AAT1 antibody
- Alanine aminotransferase 1 antibody
- Alanine aminotransferase antibody
see all
Images
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All lanes : Anti-Alanine Transaminase antibody (ab137531) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
10% SDS PAGE -
Anti-Alanine Transaminase antibody (ab137531) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 55 kDa
7.5% SDS PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137531 has not yet been referenced specifically in any publications.