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Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373)

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Overview

Product name

Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal
See all Alanyl-tRNA synthetase 2 products (2) ...

Description

Rabbit polyclonal to Alanyl-tRNA synthetase 2 - C-terminal

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide from the C-terminal region of human Alanyl-tRNA synthetase 2 conjugated to KLH

Positive control

SK-BR-3 cell line lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Ammonium Sulphate Precipitation

Purification notes

ab72373 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373)Western blot - Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Alanyl-tRNA synthetase 2 antibody - C-terminal(ab72373)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Alanyl-tRNA synthetase 2 antibody - C-terminal(ab72373) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab72373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/50 - 1/100.Detects...Read more →

    WB: 1/50 - 1/100.Detects a band of approximately 95 kDa (predicted molecular weight: 107 kDa).

  • ELISA

     ELISA: 1/1000

    ELISA: 1/1000

  • 1 Image

    IHC-P

     IHC-P: 1/10 - 1/50.

    IHC-P: 1/10 - 1/50.

Target

Relevance

Alanyl-tRNA synthetase 2 (AARS2) is an enzyme that catalyzes the chemical reaction: ATP + L-alanine + tRNA(Ala) <---> AMP + diphosphate + L-alanyl-tRNA(Ala). This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis.

Cellular localization

Mitochondrion matrix

Alternative names

  • AARSL antibody
  • bA444E17.1 antibody
  • Gm89 antibody
  • MT ALARS antibody
  • MTALARS antibody
  • AARS 2 antibody
  • AARS2 antibody
  • Alanine tRNA ligase 2 mitochondrial antibody
  • Alanine tRNA ligase antibody
  • Alanyl tRNA synthetase 2 mitochondrial antibody
  • Alanyl tRNA synthetase like antibody
  • AlaRS antibody
  • KIAA1270 antibody
  • MGC69820 antibody
  • Probable alanyl tRNA synthetase mitochondrial antibody
  • RP11-444E17.1 antibody
see all

Database links

Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal images:

  Western blot - Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373)

Western blot - Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373)

Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373) at 1/60 dilution + SK-BR-3 cell line lysate at 35 µg

Predicted band size : 107 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Alanyl-tRNA synthetase 2 antibody - C-terminal(ab72373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Alanyl-tRNA synthetase 2 antibody - C-terminal(ab72373)

ab72372 at 1/50 dilution staining Alanyl tRNA synthetase 2 C-terminal in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373)

ab72373 has not yet been referenced specifically in any publications.

Publishing research using ab72373? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"