Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Overview
Product name
Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal
See all Alanyl-tRNA synthetase 2 products (2) ...
Description
Rabbit polyclonal to Alanyl-tRNA synthetase 2 - C-terminal
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the C-terminal region of human Alanyl-tRNA synthetase 2 conjugated to KLH
Positive control
SK-BR-3 cell line lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
ab72373 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Detects a band of approximately 95 kDa (predicted molecular weight: 107 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/10 - 1/50.
Target
Relevance
Alanyl-tRNA synthetase 2 (AARS2) is an enzyme that catalyzes the chemical reaction: ATP + L-alanine + tRNA(Ala) <---> AMP + diphosphate + L-alanyl-tRNA(Ala). This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis.
Cellular localization
Mitochondrion matrix
Alternative names
- AARSL antibody
- bA444E17.1 antibody
- Gm89 antibody
see all
Database links
- Entrez Gene: 57505 Human
- Omim: 612035 Human
- SwissProt: Q5JTZ9 Human
Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal images:
Western blot - Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373)
Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373) at 1/60 dilution + SK-BR-3 cell line lysate at 35 µg
Predicted band size : 107 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Alanyl-tRNA synthetase 2 antibody - C-terminal(ab72373)
ab72372 at 1/50 dilution staining Alanyl tRNA synthetase 2 C-terminal in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373)
ab72373 has not yet been referenced specifically in any publications.
Publishing research using ab72373? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"