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Western blot - Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Alanyl-tRNA synthetase 2 antibody - C-terminal(ab72373)
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Product Name
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Alanyl-tRNA synthetase 2 antibody - C-terminal
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See all Alanyl-tRNA synthetase 2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Alanyl-tRNA synthetase 2 - C-terminal
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Immunogen
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Synthetic peptide from the C-terminal region of human Alanyl-tRNA synthetase 2 conjugated to KLH
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/10 - 1/50.
WB: 1/50 - 1/100. Detects a band of approximately 95 kDa (predicted molecular weight: 107 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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SK-BR-3 cell line lysate.
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Cellular localization
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Mitochondrion matrix
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
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Relevance
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Alanyl-tRNA synthetase 2 (AARS2) is an enzyme that catalyzes the chemical reaction: ATP + L-alanine + tRNA(Ala) <---> AMP + diphosphate + L-alanyl-tRNA(Ala). This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Ammonium Sulphate Precipitation
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab72373 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Alanyl-tRNA synthetase 2 antibody - C-terminal is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Alanyl-tRNA synthetase 2 antibody - C-terminal images, references, products related to ab72373 and other tools.
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Alanyl-tRNA synthetase 2 antibody - C-terminal images:
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 Western blot - Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373)
Alanyl-tRNA synthetase 2 antibody - C-terminal (ab72373) at 1/60 dilution + SK-BR-3 cell line lysate at 35 µg
Predicted band size : 107 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Alanyl-tRNA synthetase 2 antibody - C-terminal(ab72373)
ab72372 at 1/50 dilution staining Alanyl tRNA synthetase 2 C-terminal in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
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Search PubMed (MEDLINE) for references to Alanyl-tRNA synthetase 2
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Alanyl-tRNA synthetase 2 antibody - C-terminal - more information
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Alanyl-tRNA synthetase 2 antibody - C-terminal - related products:
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Alanyl-tRNA synthetase 2 antibody - C-terminal - other tools:
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