Anti-Alas1 antibody (ab101681)

Overview

• Product nameAnti-Alas1 antibodySee all Alas1 primary antibodies ...
• Description
  Rabbit polyclonal to Alas1
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Horse, Cow, Macaque Monkey
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Alas1.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in HepG2 nuclear whole cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Cardiovascular
  • Blood
  • Other

Applications

Target

• PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
• Sequence similaritiesBelongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
• Cellular localizationMitochondrion matrix.

Target information above from: UniProt accession P13196 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 211 Human
  • Entrez Gene: 11655 Mouse
  • Entrez Gene: 65155 Rat
  • Omim: 125290 Human
  • SwissProt: P13196 Human
  • SwissProt: Q8VC19 Mouse
  • SwissProt: P13195 Rat
  • Unigene: 476308 Human

  • Unigene: 290578 Mouse
  • Unigene: 97126 Rat

  see all

• Alternative names
  5 aminolevulinate synthase antibody5 aminolevulinate synthase nonspecific mitochondrial antibody5 aminolevulinic acid synthase antibody

  5-aminolevulinate synthase antibody5-aminolevulinic acid synthase 1 antibodyAlas 1 antibodyALAS 3 antibodyALAS antibodyALAS H antibodyALAS HOUSEKEEPING TYPE antibodyALAS N antibodyALAS-H antibodyalaS1 antibodyALAS3 antibodyALASH antibodyAminolevulinate delta synthase 1 antibodyAminolevulinic acid synthase 1 antibodyDelta ALA synthetase antibodyDelta aminolevulinate synthase antibodyDelta-ALA synthase 1 antibodyDelta-aminolevulinate synthase 1 antibodyHEM1_HUMAN antibodyMIG 4 antibodyMIG4 antibodyMigration inducing protein 4 antibodymitochondrial antibodynonspecific antibody

  see all

Anti-Alas1 antibody images

• 

  Western blot - Alas1 antibody (ab101681)
  Anti-Alas1 antibody (ab101681) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 70 kDa
  Observed band size : 70 kDa
  Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 4 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody (ab101681)
  ICC/IF image of ab101681 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101681, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab101681, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.