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Products:Cardiovascular >> Blood >> Other
Overview
Product name
Anti-Alas1 antibody
See all Alas1 products (5) ...
Description
Rabbit polyclonal to Alas1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Dog, Chimpanzee
Immunogen
Synthetic peptide: M LLAGALESVR ILKSAEGRVL RRQHQRNVKL MRQMLMDAGL PVVHCPSHII, corresponding to amino acids 480-530 of Human Alas1.
M LLAGALESVR ILKSAEGRVL RRQHQRNVKL MRQML MDAGL PVVHCPSHII
Positive control
HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Aliquot and store at -20°C or -80°C when reconstituted. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified by chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Mitochondrial
Cardiovascular >> Blood >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 3 µg/ml.Predicted molecular weight: 71 kDa.
ICC/IF: Use a concentration of 5 µg/ml
Target
Pathway
Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Sequence similarities
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP13196
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- nonspecific antibody
- 5 aminolevulinate synthase antibody
see all
Database links
- Entrez Gene: 552895 Chicken
- Entrez Gene: 211 Human
- Entrez Gene: 11655 Mouse
- Entrez Gene: 65155 Rat
- Omim: 125290 Human
- SwissProt: P07997 Chicken
- SwissProt: P13196 Human
- SwissProt: Q8VC19 Mouse
see all
Anti-Alas1 antibody images:
Western blot - Alas1 antibody (ab22153)
Anti-Alas1 antibody (ab22153) at 1 µg/ml + HepG2 cell lysate. at 20 µg
Predicted band size : 71 kDa
Immunocytochemistry/ Immunofluorescence-Alas1 antibody(ab22153)
ICC/IF image of ab22153 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22153, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Alas1 antibody (ab22153)
This product has been referenced in:
- Tian Qet al. Lon Peptidase 1 (LONP1)-dependent Breakdown of Mitochondrial 5-Aminolevulinic Acid Synthase Protein by Heme in Human Liver Cells. J Biol Chem 286:26424-30 (2011). WB.Read more (PubMed: 21659532) »
See 1 publication for this product
Publishing research using ab22153? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"