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Anti-Alas1 antibody (ab84962) at 1 µg/ml (5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg
Secondary
anti-Rabbit IgG HRP at 1/50000 dilution
Predicted band size : 71 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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