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Western blot - Alas1 antibody (ab84962)
(enlarge)
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Product Name
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Alas1 antibody
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See all Alas1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Alas1
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Immunogen
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Synthetic peptide within residues: VAETSAGPSV VSVKTDGGDP SGLLKNFQDI MQKQRPERVS HLLQDNLPKS, corresponding to N terminal amino acids 144-193 of Human Alas1 (NP_000679).
BLAST 'VAETSAGPSV VSVKTDGGDP SGLLKNFQDI MQKQRPERVS HLLQDNLPKS ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 71 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human fetal lung lysate.
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Cellular localization
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Mitochondrial matrix.
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Research areas
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Signal Transduction >> Metabolism >> Mitochondrial
Cardiovascular >> Blood >> Other
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Relevance
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Delta aminolevulinate synthase catalyzes the condensation of glycine with succinyl CoA to form delta aminolevulinic acid. This nuclear encoded mitochondrial enzyme is the first and rate limiting enzyme in the mammalian heme biosynthetic pathway.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Alas1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Alas1 antibody images, references, products related to ab84962 and other tools.
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Alas1 antibody images:
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 Western blot - Alas1 antibody (ab84962)
Alas1 antibody (ab84962) at 1 µg/ml (5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg
Secondary
anti-Rabbit IgG HRP at 1/50000 dilution
Predicted band size : 71 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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Search PubMed (MEDLINE) for references to Alas1
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Alas1 antibody - more information
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Customer reviews (feedback) regarding Alas1 antibody |
Customer FAQs regarding Alas1 antibody |
Protocols for Alas1 antibody |
Price and availability of products related to Alas1 antibody |
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Alas1 antibody - related products:
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Alas1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Alas1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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