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Products:Cardiovascular >> Blood >> Other
Overview
Product name
Anti-Alas1 antibody
See all Alas1 products (5) ...
Description
Rabbit polyclonal to Alas1
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken, Cow, Dog, Zebrafish
Immunogen
Synthetic peptide within residues: VAETSAGPSV VSVKTDGGDP SGLLKNFQDI MQKQRPERVS HLLQDNLPKS, corresponding to N terminal amino acids 144-193 of Human Alas1 (NP_000679).
VAETSAGPSV VSVKTDGGDP SGLLKNFQDI MQKQRPERVS HLLQDNLPKS
Positive control
Human fetal lung lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Mitochondrial
Cardiovascular >> Blood >> Other
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Western blot - Alas1 antibody (ab84962)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 65 kDa (predicted molecular weight: 71 kDa).
Target
Pathway
Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Sequence similarities
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP13196
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- nonspecific antibody
- 5 aminolevulinate synthase antibody
see all
Database links
- Entrez Gene: 552895 Chicken
- Entrez Gene: 211 Human
- Entrez Gene: 11655 Mouse
- Entrez Gene: 65155 Rat
- Entrez Gene: 64608 Zebrafish
- Omim: 125290 Human
- SwissProt: P07997 Chicken
- SwissProt: P13196 Human
see all
Anti-Alas1 antibody images:
Western blot - Alas1 antibody (ab84962)
Anti-Alas1 antibody (ab84962) at 1 µg/ml (5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg
Secondary
anti-Rabbit IgG HRP at 1/50000 dilution
Predicted band size : 71 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
References for Anti-Alas1 antibody (ab84962)
This product has been referenced in:
- Chernova Tet al. Early Failure of N-Methyl-D-aspartate Receptors and Deficient Spine Formation Induced by Reduction of Regulatory Heme in Neurons. Mol Pharmacol 79:844-54 (2011). WB; Mouse.Read more (PubMed: 21325018) »
See 1 publication for this product
Publishing research using ab84962? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"