Overview
- Product nameAlbumin Human ELISA kitSee all Albumin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine
- Assay typeSandwich
- Sensitivity= 3 ng/ml
- Range3.125 ng/ml - 200 ng/ml
- Recovery
99 %
- Assay time3h 00m
- Species reactivityReacts with: Human
- Product overview
Albumin is a serum hepatic protein, the most abundant protein in serum and contributes to the maintenance of oncotic pressure as well as to transport of hydrophobic molecules. Serum albumin level has been linked in clinical practice to several diseases. Low albumin levels can suggest liver, kidney disease, inflammation, shock, and malnutrition. On the other hand, high albumin levels usually reflect dehydration.
ab108788 Albumin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the detection of human albumin in urine, saliva, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human albumin in less than 3 hours. A polyclonal antibody specific for human albumin has been pre-coated onto a 96-well microplate with removable strips. Albumin in standards and samples is sandwiched by the immobilized polyclonal antibody and biotinylated polyclonal antibody specific for human albumin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.
- Tested applicationsSandwich ELISA, ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 96 Well polystyrene microplate (12 strips of 8 wells) 1 unit Biotinylated Human Albumin Antibody (50X) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Albumin Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml -
Research Areas
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Alternative names
- ALBalbuminCell growth inhibiting protein 42
- CSAGIG20GIG42Growth inhibiting protein 20PRO0883PRO0903PRO1708PRO2044PRO2619PRO2675UNQ696/PRO1341
see all
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
Applications
Our Abpromise guarantee covers the use of ab108788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
| ELISA | ELISA |
Albumin Human ELISA kit images
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ELISA - Albumin Human ELISA KitELISA analysis of Albumin in HepG2 cell conditioned media and fresh growth media containing FBS, using ab108788 Albumin Human ELISA Kit.
This image is courtesy of an anonymous Abreview. -
Sandwich ELISA - Albumin Human ELISA kit 1 x 96 Well Plate (ab108788)Standard curve
The curve provided is for illustration only. A standard curve should be generated each time the assay is performed.
Protocols
References for Albumin Human ELISA kit (ab108788)
ab108788 has not yet been referenced specifically in any publications.
