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Overview

  • Product nameAlbumin Rat ELISA KitSee all Albumin kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 0.7 µg/ml
  • Range
    0.78 µg/ml - 50 µg/ml
  • Recovery

    101 %

  • Assay time
    2h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Albumin is a serum hepatic protein, the most abundant protein in serum and contributes to the maintenance of oncotic pressure as well as to transport of hydrophobic molecules. Serum albumin level has been linked in clinical practice to several diseases. Low albumin levels can suggest liver, kidney disease, inflammation, shock, and malnutrition. On the other hand, high albumin levels usually reflect dehydration.

    ab108790 Albumin Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit employs a quantitative competitive enzyme immunoassay technique that measures rat plasma and serum albumin in less than 2 hours. A polyclonal antibody specific for rat albumin has been pre-coated onto a 96-well microplate with removable strips. Albumin in standards and samples is competed with a biotinylated Albumin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.

  • Tested applicationsCompetitive ELISA more details
  • PlatformMicroplate

Properties

  • Cellular localizationSecreted.
    • Alternative names
        ALBalbuminCell growth inhibiting protein 42
        CSAGIG20GIG42Growth inhibiting protein 20PRO0883PRO0903PRO1708PRO2044PRO2619PRO2675UNQ696/PRO1341
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108790 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Competitive ELISA cELISA

      Albumin Rat ELISA Kit images

      • ELISA - Albumin Rat ELISA Kit (ab108790)
        ELISA - Albumin Rat ELISA Kit (ab108790)
        Albumin Standard Curve

      Protocols

      References for Albumin Rat ELISA Kit (ab108790)

      ab108790 has not yet been referenced specifically in any publications.

      Publishing research using ab108790 ? Please let us know so that we can cite the reference in this datasheet

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      Inquiry: Concerning Albumin Rat ELISA Kit (ab108790), how much plasma is required per assay well? Does the assay cross react with bovine serum albumin?



      I can confirm that 25ul sample per well is used:

      The sample is plasma collected using one-tenth volume of 0.1 M sodium citrate as an anticoagulant. Centrifuge samples at 2000 x g for 10 minutes and assay. Dilute samples 1:8000 into Dil...

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      I want to know how to order the Rat Albumin Standard (150ug) and Rat Albumin Microplate (1X96 test) in the Albumin Rat Elisa Kit (ab108790) separately?

      Thank you for contacting us.

      Unfortunately we only sell the kits as complete units. These come in from the lab pre-assembled and there is no way for us to split apart kits to sell separately.

      I apologize that this information could n...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"