Overview
- Product nameAlcam Human ELISA Kit
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 15 pg/ml
- Range20.48 pg/ml - 5000 pg/ml
- Recovery
100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 110.9 101% - 118% Serum 99.33 92% - 106% Plasma 91 81% - 100% - Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
ab113317 Human Alcam ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Alcam (activated leukocyte cell adhesion molecule) in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human Alcam coated on a 96-well plate. Standards and samples are pipetted into the wells and Alcam present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Alcam antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Alcam bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Notes
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Alcam Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated anti human Alcam 2 vials Recombinant Human Alcam Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml - Research Areas
- SwissProt: Q13740 Human
Applications
Our Abpromise guarantee covers the use of ab113317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
Alcam Human ELISA Kit images
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Typical data BRepresentative standard curve using ab113317
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Typical data ARepresentative standard curve using ab113317
Protocols
References for Alcam Human ELISA Kit (ab113317)
ab113317 has not yet been referenced specifically in any publications.
