Alcohol Dehydrogenase Detection Kit (ab102533)

Overview

  • Product nameAlcohol Dehydrogenase Detection Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell culture media
  • Assay typeEnzyme activity
  • Sensitivity
    = 0.01 mU/well
  • Product overview

    Abcam's Alcohol Dehydrogenase Detection Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceAlcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
  • Cellular localizationCytoplasmic
  • Alternative names
    • ADH
    • ADH alpha subunit
    • ADH beta subunit
    • ADH1
    • ADH1A
    • ADH1B
    • ADH2
    • Alcohol dehydrogenase 1A
    • Alcohol dehydrogenase 1A (class I) alpha polypeptide
    • Alcohol dehydrogenase 1B
    • Alcohol dehydrogenase 1B (class I) beta polypeptide
    • Alcohol dehydrogenase 2 (class I) beta polypeptide
    • Alcohol dehydrogenase subunit alpha
    • Alcohol dehydrogenase subunit beta
    • Aldehyde reductase
    see all

Applications

Our Abpromise guarantee covers the use of ab102533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

Alcohol Dehydrogenase Detection Kit images

  • NADH Standard Curve
  • Sample: Bovine Liver extraction (36µg protein)

Protocols

References for Alcohol Dehydrogenase Detection Kit (ab102533)

ab102533 has not yet been referenced specifically in any publications.

Product Wall

As long as there is active Alcohol Dehydrogenase in the plant extract, our kit ab102533 should work and you will have our guarantee
This means, as the kit doesn't have a species restriction, we cannot offer you our testing discount.



I can confirm that this kit is tested and guaranteed for cell culture supernatant as well as serum.

Please treat the cell culture supernatant samples as describes for serum:

For cell culture supernatant samples, 5 – ...

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Nous n'avons malheureusement pas de données expérimentales ni de protocole recommandée quant à l'utilisation de kits enzymatiques avec des inhibiteurs. Je pense personnellement qu'une simple incubation de 10 minutes devrait être suffisante. Je recom...

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Nos collaborateurs qui produisent les kits ab83369, ab83464 et ab102533 m'ont confirmé qu'il est en effet possible d'effectuer des tests d'inhibition avec ces produits. Autre bonne nouvelle, nous avons actuellement un offre promotionnelle "3 pour 2"...

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This kit is compatible with all mammalian samples and hence will be compatible with rat samples as well. An example of blood sample preparation for the kit: Collect blood using citrate or EDTA. Centrifuge at 1,000 x g for 10 min at 4°C. Transfer...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"