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Does this antibody recognize all of the Alcohol Dehydrogenase isoenzymes? |
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ANSWER: |
Thank you for your enquiry. I can confirm that the antibody was raised to ADH1A but unfortunately we have not tested it with other isoenzymes. I hope this information helps. Please do not hesitate to contact us again if you need anything further. |
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Is this antibody specific for the alpha or gamma isoforms. |
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ANSWER: |
Unfortunately we have not tested the subtype specificity. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Ab8335 staining human normal liver. Staining is localised to the cytoplasmic compartment.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH6 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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