 image](#Alcohol-Dehydrogenase-Primary-antibodies-ab8335-1.jpg)
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Products:Signal Transduction >> Metabolism >> Alcohol metabolism
Overview
Product name
Anti-Alcohol Dehydrogenase antibody [1E5]
See all Alcohol Dehydrogenase products (9) ...
Description
Mouse monoclonal [1E5] to Alcohol Dehydrogenase
Specificity
This antibody is against human Alcohol Dehydrogenase (ADH). This antibody reacts with ADH with Kd=10-10M This antibody does not influence oxidoreductase activity
Tested applications
IHC-P, ICCmore details
Cross reactivity
Reacts with
Human
Does not react with
Rat, Rabbit
Immunogen
Purified alcohol dehydrogenase from human liver
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS with 0.1% sodium azide, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
Immunocytochemical analysis of hepatocyte differentiation.
Clonality
Monoclonal
Clone number
1E5
Myeloma
Sp2/0
Isotype
IgG1
Light chain type
unknown
Research areas
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Alcohol Dehydrogenase antibody [1E5](ab8335)
 image](/ps/datasheet/images/8/ab8335/Alcohol-Dehydrogenase-Primary-antibodies-ab8335-1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/ml
ICC: Use at an assay dependent concentration.
Target
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP07327
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADC1 antibody
- ADH alpha subunit antibody
- ADH antibody
see all
Database links
- Entrez Gene: 124 Human
- Omim: 103700 Human
- SwissProt: P07327 Human
- Unigene: 654433 Human
Anti-Alcohol Dehydrogenase antibody [1E5] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Alcohol Dehydrogenase antibody [1E5](ab8335)
Ab8335 staining human normal liver. Staining is localised to the cytoplasmic compartment.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH6 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-Alcohol Dehydrogenase antibody [1E5] (ab8335)
ab8335 has not yet been referenced specifically in any publications.
Publishing research using ab8335? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"