(2980-1)
Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)
Overview
- Product nameAnti-Alcohol Dehydrogenase antibody [EPR4440]See all Alcohol Dehydrogenase primary antibodies ...
- DescriptionRabbit monoclonal [EPR4440] to Alcohol Dehydrogenase
- Tested applicationsWB, IP, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
A synthetic peptide corresponding to residues in Human Alcohol Dehydrogenase.
- Positive controlWB: Human fetal liver, rat liver and mouse liver lysates IHC-P: Human liver tissue
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR4440
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab108197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
| IP | IP: 1/10 - 1/100. |
| IHC-P | IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
Target
- Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family.
- Cellular localizationCytoplasm.
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Database links
- Entrez Gene: 124 Human
- Entrez Gene: 11522 Mouse
- Entrez Gene: 24172 Rat
- Omim: 103700 Human
- SwissProt: P07327 Human
- SwissProt: P00329 Mouse
- SwissProt: P06757 Rat
- Unigene: 654433 Human
- Unigene: 40222 Rat
see all
Target information above from: UniProt accession
P07327
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ADH alpha subunit antibodyADH antibodyADH1 antibody
- ADH1A antibodyADH1A_HUMAN antibodyAlcohol dehydrogenase 1 antibodyAlcohol dehydrogenase 1A antibodyAlcohol dehydrogenase subunit alpha antibodyAldehyde reductase antibody
see all
Anti-Alcohol Dehydrogenase antibody [EPR4440] images
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![Western blot - Alcohol Dehydrogenase antibody [EPR4440] (ab108197)](http://a.abcam.com/ps/datasheet/images/108/ab108197/Alcohol-Dehydrogenase-Primary-antibodies-ab108197-1.jpg)
Western blot - Alcohol Dehydrogenase antibody [EPR4440] (ab108197)All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Mouse liver lysate
Lane 3 : Rat liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 40 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcohol Dehydrogenase antibody [EPR4440] (ab108197)](http://a.abcam.com/ps/datasheet/images/108/ab108197/Alcohol-Dehydrogenase-Primary-antibodies-ab108197-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcohol Dehydrogenase antibody [EPR4440] (ab108197)Immunohistochemical staining of Alcohol Dehydrogenase in paraffin embedded Human liver tissue, using ab108197 at a 1/100 dilution.
References for Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)
ab108197 has not yet been referenced specifically in any publications.
