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Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (ab211112) provides a convenient tool for sensitive detection of Aldo-Keto Reductase (AKR) activity in animal tissue and cells, as well as biological fluids such as serum. The assay is based on the ability of AKR to reduce a general substrate and convert NADP+ to NADPH, which reacts with the AKR probe to generates color that can be detected at OD 450 nm. The intensity of the color is proportional to the activity of AKR in the sample.
This assay can detect as low as 1 µU of AKR activity in samples. It has been validated with AKR1B10, AKR1C1 and AKR1C3.
Aldo-keto Reductases (AKRs) are a superfamily of NAD(P)H linked oxidoreductases which contains more than 190 members and are present in nearly all phyla. They are mainly monomeric soluble proteins (34 – 37 kD) and their common is to oxidize/reduce aldehydes or ketones to their corresponding primary or secondary alcohols. Due to their broad substrate specificity, AKRs play roles in intermediary metabolism, biosynthesis and detoxification.
In humans, AKRs are not only involved in carbonyl metabolism, but they also regulate signaling through nuclear receptors which leads to chemical carcinogenesis. Many studies demonstrate increasing expression of AKRs in cancers such as lung, liver and colon cancers.
|AKR Assay Buffer||1 x 25ml|
|AKR Substrate||1 x 15ml|
|AKR Probe||1 vial|
|AKR Positive Control||1 x 10µl|
|NADPH Standard||1 vial|
Our Abpromise guarantee covers the use of ab211112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Typical NADPH standard curve.
Kinetic curves showing AKR activity detection in increase amounts of positive control included in the kit (μL/assay).
AKR specific activity in human tissue lysates and cell line lysates. Serial dilutions were tested to ensure the readings were within the linear range of the Standard Curve.
ab211112 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"