Overview

  • Product name
  • Description
    Rabbit polyclonal to Aldolase B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment corresponding to Human Aldolase B aa 84-288.

  • Positive control
    • 293T, A431, Jurkat, Raji whole cells lysates; mouse liver lysate; methanol-fixed A431 cells; Breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab137628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.

Target

  • Pathway
    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
  • Involvement in disease
    Defects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.
  • Sequence similarities
    Belongs to the class I fructose-bisphosphate aldolase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALDB antibody
    • ALDO B antibody
    • ALDO2 antibody
    • ALDOB antibody
    • ALDOB_HUMAN antibody
    • Aldolase 2 antibody
    • Aldolase B antibody
    • Aldolase B fructose bisphosphate antibody
    • Aldolase2 antibody
    • AldolaseB antibody
    • EC 4.1.2.13 antibody
    • Fructose bisphosphate aldolase B antibody
    • Fructose-bisphosphate aldolase B antibody
    • Liver type aldolase antibody
    • Liver-type aldolase antibody
    • MS1077 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma labelling Aldolase B using ab137628 at 1/250 dilution.

  • Immunofluorescence analysis of methanol-fixed A431 cells laebelling Aldolase B using ab137628 antibody at 1/200 dilution. Lower panel represents cells costained with Hoechst 33342.

  • All lanes : Anti-Aldolase B antibody (ab137628) at 1/1000 dilution

    Lane 1 : 293T whole cells lysate
    Lane 2 : A431 whole cells lysate
    Lane 3 : Jurkat whole cells lysate
    Lane 4 : Raji whole cells lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS Page
  • Anti-Aldolase B antibody (ab137628) at 1/1000 dilution + mouse liver whole cells lysate at 30 µg

    Predicted band size: 39 kDa

References

ab137628 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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