Anti-Aldolase C antibody - Astrocyte Marker (ab87122)

Overview

  • Product nameAnti-Aldolase C antibody - Astrocyte Marker
    See all Aldolase C primary antibodies
  • Description
    Rabbit polyclonal to Aldolase C - Astrocyte Marker
  • Tested applicationsICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Chimpanzee, Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Aldolase C.

  • Positive control
    • This antibody gave a positive signal in Jurkat whole cell lysate as well as the following tissue lysates: Mouse brain; Mouse skeletal muscle; Rat cerebellum.

Properties

Applications

Our Abpromise guarantee covers the use of ab87122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-Fr 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa).

Target

Anti-Aldolase C antibody - Astrocyte Marker images

  • All lanes : Anti-Aldolase C antibody - Astrocyte Marker (ab87122) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa,71 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
  • ICC/IF image of ab87122 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87122, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 10µg/ml.
  • IHC-Fr image of Aldolase C staining on Mouse cortex sections using ab87122 (1:1000). The sections were fixed in paraformaldehyde and permeabilized using 0.4% triton x. The sections were blcoked using 50% serum for 30 mins at 24°C.

    See Abreview

References for Anti-Aldolase C antibody - Astrocyte Marker (ab87122)

This product has been referenced in:
  • Li J  et al. EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia. Nat Neurosci 16:1745-53 (2013). Read more (PubMed: 24162653) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.4% triton x
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 50% · Temperature: 24°C
Username

Ms. Lynette Foo

Verified customer

Submitted Nov 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"