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Anti-Aldolase C antibody - C-terminal (ab71294)

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Overview

Product name

Anti-Aldolase C antibody - C-terminal
See all Aldolase C products (3) ...

Description

Rabbit polyclonal to Aldolase C - C-terminal

Tested applications

IHC-P, ELISA, WBmore details

Cross reactivity

Reacts with

Human

Positive control

CEM cell line lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Ammonium Sulphate Precipitation

Purification notes

ab71294 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker

  • Western blot - Aldolase C antibody - C-terminal (ab71294)Western blot - Aldolase C antibody - C-terminal (ab71294) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Aldolase C antibody - C-terminal(ab71294)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Aldolase C antibody - C-terminal(ab71294) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab71294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/10 - 1/50.

    IHC-P: 1/10 - 1/50.

  • ELISA

     ELISA: 1/1000

    ELISA: 1/1000

  • ShowHide1 Image

    WB

     WB: 1/50 - 1/100.Detects...Read more →

    WB: 1/50 - 1/100.Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).

Target

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Sequence similarities

Belongs to the class I fructose-bisphosphate aldolase family.

Target information above from: UniProt accessionP09972 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ALDC antibody
  • ALDO C antibody
  • aldoc antibody
  • ALDOC_HUMAN antibody
  • Aldolase 3 antibody
  • Aldolase C Fructose bisphosphate antibody
  • Brain type aldolase antibody
  • Brain-type aldolase antibody
  • Fructoaldolase C antibody
  • Fructose 1 6 biphosphate triosephosphate lyase antibody
  • Fructose bisphosphate aldolase C antibody
  • Fructose-bisphosphate aldolase C antibody
see all

Database links

Anti-Aldolase C antibody - C-terminal images:

  Western blot - Aldolase C antibody - C-terminal (ab71294)

Western blot - Aldolase C antibody - C-terminal (ab71294)

Anti-Aldolase C antibody - C-terminal (ab71294) at 1/60 dilution + CEM cell line lysate at 35 µg

Predicted band size : 39 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Aldolase C antibody - C-terminal(ab71294)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Aldolase C antibody - C-terminal(ab71294)

ab71294 at 1/50 dilution staining Aldolase C C - terminal in human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-Aldolase C antibody - C-terminal (ab71294)

ab71294 has not yet been referenced specifically in any publications.

Publishing research using ab71294? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"