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Anti-Aldolase antibody (HRP) (ab34598)

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Overview

Product name

Anti-Aldolase antibody (HRP)
See all Aldolase products (11) ...

Description

Goat polyclonal to Aldolase (HRP)

Conjugation

HRP

Specificity

This antibody detects Aldolase.

Tested applications

WB, Dot Blot, ELISA, Electron Microscopymore details

Cross reactivity

Reacts with

Rabbit, Human

Immunogen

Full length Aldolase protein (Rabbit muscle).

Positive control

WB:Human A293, MCF7, NMB231 and HBL 100 cell lysates.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

This antibody was purified by a multi step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Aldolase antibody (HRP) (ab34598)Western blot - Aldolase antibody (HRP) (ab34598) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab34598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Dot Blot

     
  • ELISA

     
  • Electron Microscopy

     

Application notes

Dot: Use at an assay dependent dilution.
Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/2000.
WB: 1/1000. Predicted molecular weight: 39 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Cellular localization

Cytoplasmic

Alternative names

  • ALDA antibody
  • ALDOA antibody
  • Aldolase A antibody
  • Aldolase A fructose bisphosphatase antibody
  • Aldolase A fructose bisphosphate antibody
  • Fructose 1 6 bisphosphate triosephosphate lyase antibody
  • Fructose bisphosphate aldolase A antibody
  • Fructose bisphosphate aldolase antibody
  • Lung cancer antigen NY LU 1 antibody
  • MGC10942 antibody
  • MGC17716 antibody
  • MGC17767 antibody
  • Muscle type aldolase antibody
see all

Anti-Aldolase antibody (HRP) images:

  Western blot - Aldolase antibody (HRP) (ab34598)

Western blot - Aldolase antibody (HRP) (ab34598)

Anti-Aldolase antibody (HRP) (ab34598) at 1/1000 dilution + A293 cell lysate

Predicted band size : 39 kDa
Observed band size : 41 kDa (why is the actual band size different from the predicted?)

References for Anti-Aldolase antibody (HRP) (ab34598)

ab34598 has not yet been referenced specifically in any publications.

Publishing research using ab34598? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"