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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Aldolase antibody (HRP)
See all Aldolase products (11) ...
Description
Goat polyclonal to Aldolase (HRP)
Conjugation
HRP
Specificity
This antibody detects Aldolase.
Tested applications
WB, Dot Blot, ELISA, Electron Microscopymore details
Cross reactivity
Reacts with
Rabbit, Human
Immunogen
Full length Aldolase protein (Rabbit muscle).
Positive control
WB:Human A293, MCF7, NMB231 and HBL 100 cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This antibody was purified by a multi step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
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Western blot - Aldolase antibody (HRP) (ab34598)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Dot Blot
ELISA
Electron Microscopy
Application notes
Dot: Use at an assay dependent dilution.
Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/2000.
WB: 1/1000. Predicted molecular weight: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.
Cellular localization
Cytoplasmic
Alternative names
- ALDA antibody
- ALDOA antibody
- Aldolase A antibody
see all
Database links
- Entrez Gene: 226 Human
- Omim: 103850 Human
- SwissProt: P04075 Human
- SwissProt: Q6FI10 Human
- SwissProt: P00883 Rabbit
- Unigene: 513490 Human
Anti-Aldolase antibody (HRP) images:
Western blot - Aldolase antibody (HRP) (ab34598)
Anti-Aldolase antibody (HRP) (ab34598) at 1/1000 dilution + A293 cell lysate
Predicted band size : 39 kDa
Observed band size : 41 kDa (why is the actual band size different from the predicted?)
References for Anti-Aldolase antibody (HRP) (ab34598)
ab34598 has not yet been referenced specifically in any publications.
Publishing research using ab34598? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"