Anti-Aldolase antibody - N-terminal (ab71433)

Overview

• Product nameAnti-Aldolase antibody - N-terminalSee all Aldolase primary antibodies ...
• Description
  Rabbit polyclonal to Aldolase - N-terminal
• Tested applicationsIHC-P, ELISA, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide from the N-terminal region of human Aldolase conjugated to KLH

• Positive controlCEM cell line lysates.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityAmmonium Sulphate Precipitation
• Purification notesab71433 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

Applications

Target

• FunctionPlays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
• PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
• Involvement in diseaseDefects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
• Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.

Target information above from: UniProt accession P04075 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 226 Human
  • Omim: 103850 Human
  • SwissProt: P04075 Human
  • Unigene: 513490 Human

• Alternative names
  ALDA antibodyAldo1 antibodyALDOA antibody

  ALDOA_HUMAN antibodyAldolase A antibodyAldolase A fructose bisphosphatase antibodyAldolase A fructose bisphosphate antibodyFRUCTOALDOLASE A antibodyFructose 1 6 bisphosphate triosephosphate lyase antibodyFructose bisphosphate aldolase A antibodyFructose bisphosphate aldolase antibodyFRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A antibodyFructose-bisphosphate aldolase A antibodyGSD12 antibodyLung cancer antigen NY LU 1 antibodyLung cancer antigen NY-LU-1 antibodyMGC10942 antibodyMGC17716 antibodyMGC17767 antibodyMuscle type aldolase antibodyMuscle-type aldolase antibodyRNALDOG5 antibody

  see all

Anti-Aldolase antibody - N-terminal images

• 

  Western blot - Aldolase antibody - N-terminal (ab71433)
  Anti-Aldolase antibody - N-terminal (ab71433) at 1/60 dilution + CEM cell line lysate at 35 µg
  
  Predicted band size : 39 kDa
  Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Aldolase antibody - N-terminal(ab71433)
  ab71433 at 1/50 dilution staining Aldolase - N - terminal in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.